Nuclear magnetic resonance and imaging (2014/2015)

Course partially running (all years except the first)

Course code
4S000659
Credits
6
Coordinator
Michael Assfalg
Academic sector
CHIM/06 - ORGANIC CHEMISTRY
Language of instruction
Italian
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
teoria 5 I sem. Michael Assfalg
laboratorio 1 I sem. Michael Assfalg

Lesson timetable

I sem.
Activity Day Time Type Place Note
teoria Thursday 1:30 PM - 3:30 PM lesson Lecture Hall H  
laboratorio Tuesday 1:30 PM - 3:30 PM lesson Laboratory Gamma  
laboratorio Wednesday 1:30 PM - 3:30 PM lesson Laboratory Gamma  

Learning outcomes

Module:
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This course provides a framework of scientific concepts and a detailed overview of applications of nuclear magnetic resonance (NMR) techniques. Students learn about one of the most versatile biophysical techniques in life sciences, developing the ability to understand the principles of solution- and solid- state spectroscopy, and of MR imaging. Through presentation of the methods and discussion of case studies, students acquire the fundamentals to manage a broad spectrum of topics. These include: determining structure and dynamics of small and large biomolecules as well as of their supramolecular assemblies, assessing the quality of biotechnological products, investigating the physiological or pathological states of biomolecules, monitoring perturbations to metabolic profiles and identifying biomarkers of disease, applying medical imaging for diagnosis and staging of disease.

Syllabus

Module:
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Basic physical principles. The NMR spectrometer. The 1D experiment. NMR observables. Spin relaxation. Heteronuclear correlation. Multi-dimensional NMR spectra.
Analysis of low molecular weight compounds. Common NMR parameters. Assignment strategies. Chemical exchange. Spectral databases.
NMR in metabolomics. Analysis of complex metabolite mixtures in biofluids. Sample preparation methods. Metabolite identification. Metabolic fingerprinting. Multivariate statistical analysis. Diffusion ordered spectroscopy.
Protein NMR techniques. Isotope labelling strategies. Assessment of sample purity, folding, aggregation state. Residue-specific resonance assignment. Protein mobility. Structure determination.
Interaction studies. Protein interactions with ligands and partner proteins. Relaxation and saturation transfer methods. Chemical shift mapping and structure-activity relationship (SAR) by NMR.
In-cell NMR. Exploring biomolecules in physiological environments. Physicochemical properties of cells. Crowding and confinement effects. Intracellular protein delivery. Recombinant protein expression. Sample preparation methods. NMR in E. coli, oocytes, human cells.
Solid-state NMR. Anisotropic interactions. Magic angle spinning. Applications to membrane proteins and fibrils.
Magnetic resonance imaging. Magnetic field gradients and slice selection. Basic imaging techniques. Contrast in MRI. MRI in the clinics. Molecular MRI.

There are tutorials on the analysis of NMR spectra of small molecules and proteins, methods of transformation of the spectra, NMR experiment setting, sample preparation

Assessment methods and criteria

Module:
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The evaluation will be based on an oral discussion of the topics covered.

Teaching aids
Title Format (Language, Size, Publication date)
10 NMR di proteine  pdfpdf (it, 3681 KB, 27/10/14)
11 Marcatura isotopica  pdfpdf (it, 7423 KB, 29/10/14)
12 Determinazione strutturale  pdfpdf (it, 2999 KB, 29/10/14)
13 Mobilità proteine  pdfpdf (it, 4049 KB, 04/11/14)
14 Proteina legante  pdfpdf (it, 1394 KB, 05/11/14)
15 Proteina Legante 2  pdfpdf (it, 2404 KB, 06/11/14)
1 Materiale  pdfpdf (it, 21 KB, 07/10/14)
2 Basi fisiche  pdfpdf (it, 391 KB, 07/10/14)
3 Spostamento chimico  pdfpdf (it, 1717 KB, 13/10/14)
4 Accoppiamento scalare  pdfpdf (it, 2560 KB, 13/10/14)
5 Rilassamento  pdfpdf (it, 2283 KB, 14/10/14)
6 Strumentazione Pratica  pdfpdf (it, 1243 KB, 15/10/14)
7 Metabolomica  pdfpdf (it, 7330 KB, 20/10/14)
8 NMR 2D omonucleare  pdfpdf (it, 2026 KB, 22/10/14)
9 NMR 2D eteronucleare  pdfpdf (it, 1364 KB, 22/10/14)
DomandeTipo  docxdocx (it, 25 KB, 15/01/15)

Statistics about transparency requirements (Attuazione Art. 2 del D.M. 31/10/2007, n. 544)

Statistics
Outcomes Exams Outcomes Percentages Average Standard Deviation
Positive 100.0% 28 1
Rejected --
Absent --
Ritirati --
Canceled --
Distribuzione degli esiti positivi
18 19 20 21 22 23 24 25 26 27 28 29 30 30 e Lode
0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 0.0% 33.3% 0.0% 33.3% 0.0% 33.3% 0.0%

Data from AA 2014/2015 based on 3 students. I valori in percentuale sono arrotondati al numero intero più vicino.

Studying