Publications

Overexpression of PhEXPA1 increases cell size, modifies cell wall polymer composition and affects the timing of axillary meristem development in Petunia hybrida.  (2011)

Authors:
S. Zenoni; M. Fasoli; G. B. Tornielli; S. D. Santo; A. Sanson; P. d. Groot; S. Sordo; S. Citterio; F. Monti; M. Pezzotti
Title:
Overexpression of PhEXPA1 increases cell size, modifies cell wall polymer composition and affects the timing of axillary meristem development in Petunia hybrida.
Year:
2011
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Format:
A Stampa
Referee:
Name of journal:
New Phytologist
ISSN of journal:
0028-646X
N° Volume:
191
:
Blackwell Publishing
Page numbers:
662-677
Code PMID:
21534969
Keyword:
axillary meristem, cell expansion, cell wall, expansin, Petunia hybrida
Short description of contents:
" Expansins are cell wall proteins required for cell enlargement and cell wall loosening during many developmental processes. The involvement of the Petunia hybrida expansin A1 (PhEXPA1) gene in cell expansion, the control of organ size and cell wall polysaccharide composition was investigated by overexpressing PhEXPA1 in petunia plants. " PhEXPA1 promoter activity was evaluated using a promoter-GUS assay and the protein's subcellular localization was established by expressing a PhEXPA1-GFP fusion protein. PhEXPA1 was overexpressed in transgenic plants using the cauliflower mosaic virus (CaMV) 35S promoter. Fourier transform infrared (FTIR) and chemical analysis were used for the quantitative analysis of cell wall polymers. " The GUS and GFP assays demonstrated that PhEXPA1 is present in the cell walls of expanding tissues. The constitutive overexpression of PhEXPA1 significantly affected expansin activity and organ size, leading to changes in the architecture of petunia plants by initiating premature axillary meristem outgrowth. Moreover, a significant change in cell wall polymer composition in the petal limbs of transgenic plants was observed. " These results support a role for expansins in the determination of organ shape, in lateral branching, and in the variation of cell wall polymer composition, probably reflecting a complex role in cell wall metabolism.
Web page:
http://dx.doi.org/10.1111/j.1469-8137.2011.03726.x
Product ID:
61260
Handle IRIS:
11562/359798
Deposited On:
January 18, 2016
Last Modified:
November 2, 2016
Bibliographic citation:
S. Zenoni; M. Fasoli; G. B. Tornielli; S. D. Santo; A. Sanson; P. d. Groot; S. Sordo; S. Citterio; F. Monti; M. Pezzotti, Overexpression of PhEXPA1 increases cell size, modifies cell wall polymer composition and affects the timing of axillary meristem development in Petunia hybrida. «New Phytologist» , vol. 1912011pp. 662-677

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Studi mediante microspettroscopia FTIR di metabolismo di parete in piante e cellule vegetali Department Informatica Francesca Monti
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