Biocrystallography - Laboratorio (2007/2008)

Course partially running

Course code
4S00160
Name of lecturer
Massimiliano Perduca
Number of ECTS credits allocated
1
Academic sector
BIO/11 - MOLECULAR BIOLOGY
Language of instruction
Italian
Period
2° Sem dal Mar 3, 2008 al Jun 6, 2008.

To show the organization of the course that includes this module, follow this link * Course organization

Lesson timetable

Learning outcomes

The Biocrystallography laboratory introduces the students into the fundamentals of the field, particularly biological crystal growth. In addition to coveringh the practical aspects of protein crystal preparation, a diffraction data is collected and a protein structure is solved.

Syllabus

The Biocrystallography practical lessons entail a series of meetings focused on the following arguments:
1) An introduction covering macromolecular crystallization theory and the most used techniques.
2) Preparation of protein crystals using Microdialysis, Hanging and Sitting drop.
3) Collection of a dataset with a rotating anode conventional source, an image plate detector and a low temperature cooling system.
4) Data processing and analysis.
5) Solution of a protein structure using molecular replacement techniques.
6) Protein model building and refinement.
7) Presentation of the most widely used programs for molecular graphics.

Assessment methods and criteria

This topics are part of the Biocrystallography oral exam

Studying