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Course organization
Fundamentals of applied methodology for protein analysis.
Protein purification. Cell rupture. Protein solubilisation methods.
Colorimetric and spectroscopic methods for protein quantification.
Spectroscopic and spectrofluorimetric methods for tertiary and quaternaly structure analysis.
Chromatographic methods for protein purification. Ionic exchange, size exclusion, affiity, methal chelate, covalent.
Electrophoresis for protein purification. Polyacrylamide gel. SDS electrohoresis. Porosity gradient gel. Isoelectrofocusing. Two-diemnsional electrophoresis. Western blotting. Staining of gels. Gel electrophoresis of DNA. CApillary electrophoresis of proteins, peptides and DNA.
| Author | Title | Publisher | Year | ISBN | Note |
| J. Walker, K. Wilson | Metodologia biochimica. Le bioscienze e le biotecnologie in laboratorio. (Edizione 5) | Raffaello Cortina Editore | 2001 | 8870786870 | Presenti due copie nella biblioteca di Facoltà |
Oral exam
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