Chemistry and Analytical Biochemistry (2006/2007)

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Course code
4S00174
Credits
7
Coordinator
Massimiliano Perduca
Teaching is organised as follows:
Unit Credits Academic sector Period Academic staff
Biochimica analitica - teoria 2 BIO/10-BIOCHEMISTRY 2° Sem Massimiliano Perduca
Laboratorio di biochimica analitica 1 See the unit page See the unit page
Chimica analitica - teoria 3 CHIM/01-ANALYTICAL CHEMISTRY 2° Sem Massimiliano Perduca
Laboratorio di chimica analitica 1 See the unit page See the unit page

Learning outcomes

Module: Biochimica analitica - teoria
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Fundamentals of applied methodology for protein analysis.


Module: Laboratorio di biochimica analitica
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The practical lessons are aimed at the acquisition of manual skills on protein purification and quantitation.


Module: Chimica analitica - teoria & laboratorio
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The aim of the Analytical Chemistry Module is to give the students the basical knowledge on the instrumental analytical techniques of widest use, together with the working principles of the equipment used. It also intend to give a good level of confidence in the use of common laboratory instruments, used in food, environmental and industrial analysis.

Syllabus

Module: Biochimica analitica - teoria
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Protein purification. Cell rupture. Protein solubilisation methods.
Colorimetric and spectroscopic methods for protein quantification.
Spectroscopic and spectrofluorimetric methods for tertiary and quaternaly structure analysis.

Chromatographic methods for protein purification. Ionic exchange, size exclusion, affiity, methal chelate, covalent.

Electrophoresis for protein purification. Polyacrylamide gel. SDS electrohoresis. Porosity gradient gel. Isoelectrofocusing. Two-diemnsional electrophoresis. Western blotting. Staining of gels. Gel electrophoresis of DNA. CApillary electrophoresis of proteins, peptides and DNA.


Module: Laboratorio di biochimica analitica
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Detemrination of protein concentration.
Gel permeation.
Two-dimensional electrophoresis.


Module: Chimica analitica - teoria
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Introduction
Analytical techniques. Signal and rumor. Statistic analysis and data treatment.

Spectrophotometric analysis
Spectroscopic techniques. Absorption spectrophotometric analysis. Spectrophotometric titrations. UV-visible spectrophotometry. IR spectroscopy. FTIR spectrophotometer. Fluorescence and phosphorescence. Spectrofluorimeters.

Mass spectrometry
Instrument, desorption techniques, analyzers and detectors. MS MS techniques. Some examples of mass spectra from small an macro molecules.

Chromatographic methods of analysis
Chromatography principles. Equilibrium extraction with solvents. Chromatographic parameters and column efficiency. Qualitative and quantitative analysis. TLC. Gas chromatography principles and instrumentation. Principles and equipments for column chromatography. Absorption chromatography. Normal and inverse phase liquid chromatography. high performance liquid chromatography.


Module: Laboratorio di chimica analitica
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The Analytical Chemistry laboratory course lasts 3 half days during which 3 types of experiments are performed:

1) Determination of the nitrate content of water using spectrophotometric techniques.
2) Determination of the isosbestic point of an acid-base compound using visible spectrophotometry.
3) Photosynthetic pigment extraction from spinach leaves using chromatographic techniques.

Using the computer facilities present in the Analytical Chemistry Laboratory, the data collected during the experiments will be used to prepare the final report.

Assessment methods and criteria

Module: Biochimica analitica - teoria
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Oral exam


Module: Laboratorio di biochimica analitica
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A final report must be prepared by the students before they are allowed to take the final oral examination in Analytical Biochemistry.


Module: Chimica analitica - teoria
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Oral exam


Module: Laboratorio di chimica analitica
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A final report must be prepared by the students before they are allowed to take the final oral examination in Analytical Chemistry.

Reference books
Author Title Publisher Year ISBN Note
J. Walker, K. Wilson Metodologia biochimica. Le bioscienze e le biotecnologie in laboratorio. (Edizione 5) Raffaello Cortina Editore 2001 8870786870 Presenti due copie nella biblioteca di Facoltà
RUBINSON Kenneth A , RUBINSON Judith F CHIMICA ANALITICA STRUMENTALE (Edizione 1) Zanichelli 2002 8808-08959 Presenti due copie nella biblioteca di Facoltà

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