Biochemistry & Molecular Biology

Alessandra Astegno
Assistant Professor
Matteo Ballottari
Associate Professor
Stefano Capaldi
Assistant Professor
Massimo Crimi
Associate Professor
Alejandro Giorgetti
Assistant Professor
Massimiliano Perduca
Assistant Professor
Skills
Topic People Description ISI-CRUI
Biochemistry & Biophysics Alessandra Astegno
Matteo Ballottari
Comprensione delle relazioni struttura, dinamica e funzione di proteine
Bioinformatics Alejandro Giorgetti
The main interests and the principal activities of the area include: protein modeling from sequence, in particular using comparative modeling techniques; protein structure analysis; protein sequence analysis; protein design; the development of tools aimed at the investigation and understanding of the relationship between protein sequence, structure and function, in particular in systems that include membrane proteins. Biochemistry & Biophysics
Biochimica Fisica Daniele Dell'Orco
Physical and Systems Biochemistry, nanobiophysics, molecular basis of retinal diseases, protein-nanodevice interactions, structure-function properties in protein complexes, neuronal calcium sensors, bioinformatics and systems biology Biochemistry & Biophysics
Clinical molecular biology. Martina Montagnana
Gian Luca Salvagno
This topic includes the use of molecular biology techniques in the clinical context. Molecular Biology & Genetics
Enzymology and Protein chemistry Mariarita Bertoldi
studio della chimica delle proteine e dei meccanismi di funzionamento degli enzimi
Genome editing Donato Zipeto
Sviluppo di protocolli di genome editing mediante CRISPR/Cas9 e di linee cellulari knock-out; analisi del ruolo della β2-microglobulina nell’eziologia di malattie neurodegenerative;
Interazioni virus-cellula Donato Zipeto
Studio del ruolo del sistema MHC-I nel controllo dell’infezione da HIV
Laboratorio di Biologia e Biochimica Massimo Crimi
Role of lipids in apoptosis and in signalling pathways. Apoptosis is an active and tightly regulated form of cell death, which can also be considered a stress-induced process of cellular communication. Recent studies reveal that the lipid network within cells is involved in the regulation and propagation of death signalling. Despite the vast growth of our current knowledge on apoptosis, little is known of the specific role played by lipid molecules in the central event of apoptosis - the piercing of mitochondrial membranes. Our research focus on the earliest changes that physiological apoptosis induces in mitochondrial membranes to understand the role of mitochondrial lipids in apoptosis and discover new stress sensing mechanisms in cells. Another aspect that we are studying is the lipid signalling pathway in plants and the role played by Lipid Transfer Proteins (LTPs). Developmental changes requires a complex set of signals and lipid-derived molecules are now investigated as important metabolites. Lipid transfer proteins (LTPs) are a group of cystine-rich proteins named after their ability to bind and facilitate lipid transfer between membranes in vitro. Several studies have demonstrated that plant LTPs possess antimicrobial activity in vitro and are induced during pathogen infection, suggesting that these proteins can act in the protection against pathogens. LTPs seem to be implicated in the symbiotic association between rhizobia and leguminous plants as well. We are currently studying the role played by specific LTPs (like MtN5) in plant signalling pathways. Lipid derived molecules that can be recognised by LTPs will be investigated in the future to clarify the signal transduction pathway mediated by the members of this new class of LTPs, the Signaling LTPs. Biology
Molecular mechanisms of the antitumoral activity of various compounds Marta Palmieri
Innovative strategies to inhibit prolifaration of pancreatic adenocarcinoma in in vitro and in vivo models
Meccanismi molecolari di chemioresistenza in cellule di cancro del pancreas Massimo Donadelli
Strategie innovative per inibire la proliferazione cellulare dell'adenocarcinoma pancreatico in modelli cellulari e animali. Meccanismi molecolari oncogenici indotti da p53 mutata. Regolazione trascrizionale mediante modificazioni epigenetiche; regolazione dello stress ossidativo e dei meccanismi molecolari dell'autofagia nel cancro.
Medicina di laboratorio - biochimica clinica - analisi ematochimiche - medicina traslazionale - biomarcatori Giuseppe Lippi
Medicina di laboratorio - biochimica clinica - analisi ematochimiche - medicina traslazionale - biomarcatori
Oxidative stress and inflammation Sofia Giovanna Mariotto
- Functional cross-talk between constitutive and nitric oxide synthase. Modulation of NO production in in vitro inflammatory models. - Molecular mechanism of apoptotic activity of natural compounds towards tumoral cells. - Oxidative stress and post-translational modifications of the transcription factor STATs.
Structural Molecular Biology Stefano Capaldi
Ugo Luigi Monaco
Massimiliano Perduca
Studi di correlazione struttura-funzione di proteine mediante la diffrazione di raggi X, la Surface Plasmon Resonance (SPR) e tecniche calorimetriche (ITC-DSC), incentrandosi su proteine che hanno la proprietà di legare piccole molecole idrofobiche.
Struttura e funzione di aggregati proteici Giovanni Gotte
Studi strutturali e funzionali di multimeri e oligomeri proteici, in paricolare di varie ribonucleasi di tipo pancreatico, ottenibili tramite modifiche chimiche e/o cross linking, o attraverso aggregazione proteica tramite scambio tridimensionale di domini.
Trasduzione del segnale - biosintesi di proteine secrete Elio Maria Liboi
Glicosilazione e vie di segnalazione dei recettori ad attività tirosino chinasi nel pathway secretorio.
Methods validation in clinical chemistry Elisa Danese

Activities

Research facilities