Publications

Authors:

Vannozzi, Alessandro; Donnini, Silvia; Vigani, Gianpiero; Corso, Massimiliano; Valle, Giorgio; Vitulo, Nicola; Bonghi, Claudio; Zocchi, Graziano; Lucchin, Margherita

Title:

Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions

Year:

2017

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

FRONTIERS IN PLANT SCIENCE

ISSN of journal:

1664-462X

N° Volume:

7

Page numbers:

1994-2010

Keyword:

Vitis; ferrome; mRNA-Seq; micronutrients; strategy I

Short description of contents:

Iron chlorosis is a serious deficiency that affects orchards and vineyards reducing quality and yield production. Chlorotic plants show abnormal photosynthesis and yellowing shoots. In grapevine iron uptake and homeostasis are most likely controlled by a mechanism known as "Strategy I," characteristic of non-graminaceous plants and based on a system of soil acidification, iron reduction and transporter-mediated uptake. Nowadays, grafting of varieties of economic interest on tolerant rootstocks is widely used practice against many biotic and abiotic stresses. Nevertheless, many interspecific rootstocks, and in particular those obtained by crossing exclusively non-vinifera genotypes, can show limited nutrient uptake and transport, in particular for what concerns iron. In the present study, 101.14, a commonly used rootstock characterized by susceptibility to iron chlorosis was subjected to both Fe-absence and Fe-limiting conditions. Grapevine plantlets were grown in control, Fe-deprived, and bicarbonate-supplemented hydroponic solutions. Whole transcriptome analyses, via mRNA-Seq, were performed on root apices of stressed and unstressed plants. Analysis of differentially expressed genes (DEGs) confirmed that Strategy I is the mechanism responsible for iron uptake in grapevine, since many orthologs genes to the Arabidopsis "ferrome" were differentially regulated in stressed plant. Molecular differences in the plant responses to Fe absence and presence of bicarbonate were also identified indicating the two treatments are able to induce response-mechanisms only partially overlapping. Finally, we measured the expression of a subset of genes differentially expressed in 101.14 (such as IRT1, FERRITIN1, bHLH38/39) or known to be fundamental in the "strategy I" mechanism (AHA2 and FRO2) also in a tolerant rootstock (M1) finding important differences which could be responsible for the different degrees of tolerance observed.

Product ID:

97711

Handle IRIS:

11562/963651

Last Modified:

November 11, 2022

Bibliographic citation:

Vannozzi, Alessandro; Donnini, Silvia; Vigani, Gianpiero; Corso, Massimiliano; Valle, Giorgio; Vitulo, Nicola; Bonghi, Claudio; Zocchi, Graziano; Lucchin, Margherita, Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions «FRONTIERS IN PLANT SCIENCE» , vol. 7 , 2017 , pp. 1994-2010

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo