Publications

Authors:

Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola; Forcato, Claudio; D'Angelo, Michela; Schiavon, Riccardo; Vezzi, Alessandro; Giacometti, Giorgio Mario; Morosinotto, Tomas; Valle, Giorgio

Title:

Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion

Year:

2014

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

MOLECULAR PLANT

ISSN of journal:

1674-2052

N° Volume:

7

Number or Folder:

2

Page numbers:

323-335

Keyword:

Nannochloropsis; SOLiD; biofuel; chromosomes; de novo assembly; genome; lipids; nitrogen deprivation; telomeres.; transcriptome; Chromosomes; Gene Expression Regulation; Molecular Sequence Annotation; Nitrogen; Stramenopiles; Gene Expression Profiling; Genome

Short description of contents:

Nannochloropsis is rapidly emerging as a model organism for the study of biofuel production in microalgae. Here, we report a high-quality genomic assembly of Nannochloropsis gaditana, consisting of large contigs, up to 500 kbp long, and scaffolds that in most cases span the entire length of the chromosomes. We identified 10646 complete genes and characterized possible alternative transcripts. The annotation of the predicted genes and the analysis of cellular processes revealed traits relevant for the genetic improvement of this organism such as genes involved in DNA recombination, RNA silencing, and cell wall synthesis. We also analyzed the modification of the transcriptional profile in nitrogen deficiency-a condition known to stimulate lipid accumulation. While the content of lipids increased, we did not detect major changes in expression of the genes involved in their biosynthesis. At the same time, we observed a very significant down-regulation of mitochondrial gene expression, suggesting that part of the Acetyl-CoA and NAD(P)H, normally oxidized through the mitochondrial respiration, would be made available for fatty acids synthesis, increasing the flux through the lipid biosynthetic pathway. Finally, we released an information resource of the genomic data of N. gaditana, available online at www.nannochloropsis.org.

Product ID:

97719

Handle IRIS:

11562/963626

Last Modified:

November 16, 2022

Bibliographic citation:

Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola; Forcato, Claudio; D'Angelo, Michela; Schiavon, Riccardo; Vezzi, Alessandro; Giacometti, Giorgio Mario; Morosinotto, Tomas; Valle, Giorgio, Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion «MOLECULAR PLANT» , vol. 7 , n. 2 , 2014 , pp. 323-335

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