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Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study  (2016)

Authors:
Salvetti, Elisa; Orrù, Luigi; Capozzi, Vittorio; Martina, Alessia; Lamontanara, Antonella; Keller, David; Cash, Howard; Felis, Giovanna; Cattivelli, Luigi; Torriani, Sandra; Spano, Giuseppe
Title:
Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study
Year:
2016
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Format:
A Stampa
Referee:
Name of journal:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN of journal:
0175-7598
N° Volume:
100
Number or Folder:
10
Page numbers:
4595-4605
Keyword:
Antibiotic resistance; Bacillus coagulans; Genomics; Probiotics; Safety; Virulence factors
Short description of contents:
Probiotics are microorganisms that confer beneficial effects on the host; nevertheless, before being allowed for human consumption, their safety must be verified with accurate protocols. In the genomic era, such procedures should take into account the genomic-based approaches. This study aims at assessing the safety traits of Bacillus coagulans GBI-30, 6086 integrating the most updated genomics-based procedures and conventional phenotypic assays. Special attention was paid to putative virulence factors (VF), antibiotic resistance (AR) genes and genes encoding enzymes responsible for harmful metabolites (i.e. biogenic amines, BAs). This probiotic strain was phenotypically resistant to streptomycin and kanamycin, although the genome analysis suggested that the AR-related genes were not easily transferrable to other bacteria, and no other genes with potential safety risks, such as those related to VF or BA production, were retrieved. Furthermore, no unstable elements that could potentially lead to genomic rearrangements were detected. Moreover, a workflow is proposed to allow the proper taxonomic identification of a microbial strain and the accurate evaluation of risk-related gene traits, combining whole genome sequencing analysis with updated bioinformatics tools and standard phenotypic assays. The workflow presented can be generalized as a guideline for the safety investigation of novel probiotic strains to help stakeholders (from scientists to manufacturers and consumers) to meet regulatory requirements and avoid misleading information.
Product ID:
95186
Handle IRIS:
11562/955411
Last Modified:
December 4, 2019
Bibliographic citation:
Salvetti, Elisa; Orrù, Luigi; Capozzi, Vittorio; Martina, Alessia; Lamontanara, Antonella; Keller, David; Cash, Howard; Felis, Giovanna; Cattivelli, Luigi; Torriani, Sandra; Spano, Giuseppe, Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study «APPLIED MICROBIOLOGY AND BIOTECHNOLOGY» , vol. 100 , n. 102016pp. 4595-4605

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Realizzazione di una innovativa pasta alimentare funzionale arricchita di componenti bioattivi e probiotic (PASS-WORLD –Pasta e salute nel mondo) Department Biotecnologie Sandra Torriani
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