Publications

Authors:

Mainente, Federica; Zoccatelli, Gianni; Lorenzini, Marilinda; Cecconi, Daniela; S., Vincenzi; Rizzi, Corrado; Simonato, Barbara

Title:

Red wine proteins: Two dimensional (2-D) electrophoresis and mass spectrometry analysis

Year:

2014

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Food Chemistry

ISSN of journal:

0308-8146

N° Volume:

164

:

Elsevier

Page numbers:

413-417

Keyword:

Red wine proteins; Wine proteins precipitation; two-dimensional electrophoresis; mass spectrometry.

Short description of contents:

The aim of the present study was to optimize protein extraction from red wine (cv. Cabernet) in order to obtain a separation by two-dimensional electrophoresis (2-DE) compatible with mass spectrometry identification.Proteins were denatured by sodium dodecyl-sulphate (SDS) and precipitated as potassium salts. The potassium-DS (KDS) protein complexes obtained were treated with different solutions in order to remove the detergent. Proteins were solubilized with different buffers and separated by different electrophoretic approaches [native, urea, acid urea PAGEs and isoelectric focusing (IEF)] as the first-dimension (1-DE). The best 2D separation was achieved by using 10% saccharose in the DS removal step, and 6-cyclohexylhexyl β-d-maltoside detergent in the solubilisation buffer combined with the IEF approach. Several well focalized protein spots were obtained and analyzed through mass-spectrometry.

Product ID:

81903

Handle IRIS:

11562/743961

Deposited On:

June 19, 2014

Last Modified:

November 15, 2022

Bibliographic citation:

Mainente, Federica; Zoccatelli, Gianni; Lorenzini, Marilinda; Cecconi, Daniela; S., Vincenzi; Rizzi, Corrado; Simonato, Barbara, Red wine proteins: Two dimensional (2-D) electrophoresis and mass spectrometry analysis «Food Chemistry » , vol. 164 , 2014 , pp. 413-417

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo