Publications

Authors:

Bellisola, Giuseppe; DELLA PERUTA, Marco; Vezzalini, Marzia; Moratti, Elisabetta; Vaccari, L.; Birarda, G.; Piccinini, M.; Cinque, G.; Sorio, Claudio

Title:

Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy

Year:

2010

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Analyst

ISSN of journal:

0003-2654

N° Volume:

135

Number or Folder:

12

Page numbers:

3077-3086

Keyword:

infrared spectroscopy; FTIR; chronic myeloid leukemia; tyrosine phosphorylation; BCR/ABL

Short description of contents:

Aimed at developing accurate, reliable and cost-saving analytical techniques for drugs screening we evaluated the potential of Fourier Transform (FT) InfraRed (IR) microspectroscopy (microFTIR) as a quantitative pre-diagnostic approach for the rapid identification of IR signatures of drugs targeting specific molecular pathways causing Chronic Myeloid Leukemia (CML). To obtain reproducible FTIR absorbance spectra at the necessary spatial resolution we optimized sample preparation and acquisition parameters on a single channel Mercury-Cadmium-Telluride (MCT) detector in the spectral interval of frequencies from 4000 to 800 cm1. Single K562 cells were illuminated by Synchrotron Radiation (SR) and a number of 15 K562 cells spread in monolayer were illuminated by a conventional IR source (Globar), respectively. Combining IR spectral data with the results of complementary biochemical investigations carried out in samples by different analytical methods we identified and cross-validated IR signatures of drugs targeting the oncogenic protein BCR/ABL and its associated abnormal tyrosine kinase activity in K562 cell line. Unsupervised pattern recognition performed by Hierarchical Cluster Analysis (HCA) clustered the spectra of single K562 cells in two distinct groups roughly corresponding to living and to apoptotic cells, respectively. The corresponding IR spectral profiles were assumed to represent drug-resistant and drug-sensitive cells. Significant variations with increasing percentages of apoptotic cells were observed after the treatment of K562 cells with drugs that directly or indirectly target BCR/ABL. In conclusion, we suggest that microFTIR associated with multivariate data analysis may be useful to assess drug compounds in ex vivo cancer cell models and possibly peripheral blast cells from CML patients.

Product ID:

58243

Handle IRIS:

11562/345315

Deposited On:

March 30, 2012

Last Modified:

November 15, 2022

Bibliographic citation:

Bellisola, Giuseppe; DELLA PERUTA, Marco; Vezzalini, Marzia; Moratti, Elisabetta; Vaccari, L.; Birarda, G.; Piccinini, M.; Cinque, G.; Sorio, Claudio, Tracking InfraRed signatures of drugs in cancer cells by Fourier Transform microspectroscopy «Analyst» , vol. 135 , n. 12 , 2010 , pp. 3077-3086

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