Publications

Authors:

Burattini, Emilio; Cavagna, Matteo; R., Dell'Anna; F., Malvezzi Campeggi; Monti, Francesca; Rossi, Franca; Torriani, Sandra

Title:

A FTIR microspectroscopy study of autolysis in cells of the wine yeast Saccharomyces cerevisiae

Year:

2008

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

Sì

Name of journal:

VIBRATIONAL SPECTROSCOPY

ISSN of journal:

0924-2031

N° Volume:

47

Number or Folder:

2

Page numbers:

139-147

Keyword:

FTIR microspectroscopy, Saccharomyces cerevisiae, autolysis, wine, multivariate statistical analysis

Short description of contents:

The present paper reports the first application of FTIR microspectroscopy in the mid-infrared range to study the major biochemical changes associated with autolysis in yeast cells. Measurements were done both in transmission and in attenuated total reflection (ATR) mode on cells of Saccharomyces cerevisiae strain EC1118 before and after induction of the autolytic process in a model wine medium and in a Chardonnay base wine, Unsupervised multivariate statistical analysis (Hierarchical Cluster Analysis and Principal Component Analysis), as well as accurate spectral analysis based on curve fitting, were applied to the acquired spectra. The spectral behaviour of S. cerevisiae in the model and base wines was found to be the same. A detailed interpretation of absorption bands was given by reference to the literature and through comparison of transmission and ATR spectra. It was shown that FTIR microspectroscopy is a rapid and accurate tool to simultaneously probe the major biochemical events associated with the autolytic process. Moreover, the intrinsically higher sensitivity of ATR with respect to transmission spectra in analyzing autolysis was also demonstrated.

Product ID:

41068

Handle IRIS:

11562/317431

Deposited On:

March 21, 2012

Last Modified:

November 15, 2022

Bibliographic citation:

Burattini, Emilio; Cavagna, Matteo; R., Dell'Anna; F., Malvezzi Campeggi; Monti, Francesca; Rossi, Franca; Torriani, Sandra, A FTIR microspectroscopy study of autolysis in cells of the wine yeast Saccharomyces cerevisiae «VIBRATIONAL SPECTROSCOPY» , vol. 47 , n. 2 , 2008 , pp. 139-147

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