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Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin (PDO) Italian cheese.  (2007)

Authors:
POLI A.; GUGLIELMINI E.; SEMBENI S.; SPIAZZI M.; DELLAGLIO F.; ROSSI F.; TORRIANI S.
Title:
Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin (PDO) Italian cheese.
Year:
2007
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Referee:
Name of journal:
Letters Applied Microbiology
ISSN of journal:
0266-8254
N° Volume:
45
Number or Folder:
5
:
blackwell synergy
Page numbers:
529-534
Keyword:
Food safety, Multiplex PCR, PDO cheese, Real-time PCR detection, SE genes diffusion, Staphylococcus aureus
Short description of contents:
Aims: To evaluate the risk associated with the load and enterotoxigenicity of Staphylococcus aureus in Monte Veronese, a PDO (Protected Designation of Origin) cheese of the Lessinia area (Verona, Italy). Methods and Results: Staphylococcus aureus was quantified by a conventional culture method and by a nucA targeted real-time PCR assay developed in this study. Staphylococcus aureus numbers in cheese were higher than the limit tolerated by the Italian food legislation in 78% instances, according to both detection methods. Multiplex PCR tests for 17 Staph. aureus enterotoxin (SE) genes were applied to nucleic acids extracted from curds, cheeses and Staph. aureus isolates. The SE gene diversity appeared reduced after ripening. The gene encoding SED was found most frequently in dairy samples and the enterotoxin genes ser, sed, seg and sem predominated in the isolates. Conclusions: The occurrence of enterotoxigenic Staph. aureus strains with complex SE genotypes in this PDO cheese at numbers often exceeding the Italian tolerance threshold represents an important risk factor. Significance and Impact of the Study: The high frequency of contamination of Monte Veronese PDO cheese and, expectedly, similar typical productions from raw milk, by enterotoxigenic Staph. aureus imposes a tighter hygienic control in the earlier manufacturing phases.
Product ID:
36990
Handle IRIS:
11562/307782
Deposited On:
July 6, 2012
Last Modified:
December 4, 2019
Bibliographic citation:
POLI A.; GUGLIELMINI E.; SEMBENI S.; SPIAZZI M.; DELLAGLIO F.; ROSSI F.; TORRIANI S., Detection of Staphylococcus aureus and enterotoxin genotype diversity in Monte Veronese, a Protected Designation of Origin (PDO) Italian cheese. «Letters Applied Microbiology» , vol. 45 , n. 52007pp. 529-534

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