Publications

Authors:

Bianchini, Stefano; Bovio, Federica; Negri, Stefano; Guzzo, Flavia; Forcella, Matilde; Fusi, Paola

Title:

Gratiola officinalis Alcoholic Extract Targets Warburg Effect, Apoptosis and Cell Cycle Progression in Colorectal Cancer Cell Lines

Year:

2025

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN of journal:

1422-0067

N° Volume:

26

Number or Folder:

5

Page numbers:

1-25

Keyword:

colorectal cancer; Gratiola officinalis; verbascoside; glycolysis; energy metabolism

Short description of contents:

Colorectal cancer (CRC) is the second deadliest cancer in the Western world. Increased body weight, a diet rich in red meat and alcohol, as well as a sedentary lifestyle, are all involved in sporadic CRC pathogenesis. Since current CRC therapies show several side effects, there is a need to find new and more effective therapeutic approaches, allowing conventional drug dosages and toxicity to be reduced. Gratiola officinalis alcoholic extract was characterized by LC-MS and its effect investigated on a healthy colon mucosa cell line and on different colorectal cancer cell lines. Cell viability, apoptosis and cell cycle progression were evaluated through flow cytometry; energy production and glycolysis were investigated using Seahorse technology, while cancer markers were analyzed through Western blotting. The untargeted metabolomics analysis of G. officinalis alcoholic extract revealed glycosides of different polyphenols and glycosides of cucurbitane-type triterpenes. This extract showed a stronger impact on CRC cell line viability compared to healthy colon cells. In the E705 CRC cell line, it induced cell apoptosis and caused the downregulation of glycolysis, inhibiting cell proliferation. On the other hand, SW480 CRC cells treated with G. officinalis extract showed G2/M cell cycle arrest. This work shows that G. officinalis extract can reduce glycolysis and promote cell cycle arrest in CRC cells, suggesting that G. officinalis could represent a novel player in the prevention and treatment of CRC.

Product ID:

145860

Handle IRIS:

11562/1162448

Last Modified:

May 18, 2025

Bibliographic citation:

Bianchini, Stefano; Bovio, Federica; Negri, Stefano; Guzzo, Flavia; Forcella, Matilde; Fusi, Paola, Gratiola officinalis Alcoholic Extract Targets Warburg Effect, Apoptosis and Cell Cycle Progression in Colorectal Cancer Cell Lines «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 26 , n. 5 , 2025 , pp. 1-25

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