Publications

Authors:

Boscari, Elisa; Palle, Stefano Dalle; Vitulo, Nicola; Scapolatiello, Annalisa; Schiavon, Luca; Cariani, Alessia; Papetti, Chiara; Zane, Lorenzo; Marino, Ilaria Anna Maria; Congiu, Leonardo

Title:

MIPs: multi-locus intron polymorphisms in species identification and population genomics

Year:

2024

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

SCIENTIFIC REPORTS

ISSN of journal:

2045-2322

N° Volume:

14

Number or Folder:

1

Page numbers:

1-14

Keyword:

Forensic identification; High-throughput DNA sequencing; Molecular markers; Multiple-SNP haplotypes; Non-model organisms; Teleost fishes

Short description of contents:

: The study of species groups in which the presence of interspecific hybridization or introgression phenomena is known or suspected involves analysing shared bi-parentally inherited molecular markers. Current methods are based on different categories of markers among which the classical microsatellites or the more recent genome wide approaches for the analyses of thousands of SNPs or hundreds of microhaplotypes through high throughput sequencing. Our approach utilizes intron-targeted amplicon sequencing to characterise multi-locus intron polymorphisms (MIPs) and assess genetic diversity. These highly variable intron regions, combined with inter-specific transferable loci, serve as powerful multiple-SNP markers potentially suitable for various applications, from species and hybrid identification to population comparisons, without prior species knowledge. We developed the first panel of MIPs highly transferable across fish genomes, effectively distinguishing between species, even those closely related, and populations with different structures. MIPs offer versatile, hypervariable nuclear markers and promise to be especially useful when multiple nuclear loci must be genotyped across different species, such as for the monitoring of interspecific hybridization. Moreover, the relatively long sequences obtained ease the development of single-locus PCR-based diagnostic markers. This method, here demonstrated in teleost fishes, can be readily applied to other taxa, unlocking a new source of genetic variation.

Product ID:

140874

Handle IRIS:

11562/1134486

Last Modified:

December 19, 2024

Bibliographic citation:

Boscari, Elisa; Palle, Stefano Dalle; Vitulo, Nicola; Scapolatiello, Annalisa; Schiavon, Luca; Cariani, Alessia; Papetti, Chiara; Zane, Lorenzo; Marino, Ilaria Anna Maria; Congiu, Leonardo, MIPs: multi-locus intron polymorphisms in species identification and population genomics «SCIENTIFIC REPORTS» , vol. 14 , n. 1 , 2024 , pp. 1-14

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo