Publications

Authors:

Bongianni, M.; Catalan, M.; Perra, D.; Fontana, E.; Janes, F.; Bertolotti, C.; Sacchetto, L.; Capaldi, S.; Tagliapietra, M.; Polverino, P.; Tommasini, V.; Bellavita, G.; Kachoie, E. A.; Baruca, R.; Bernardini, A.; Valente, M.; Fiorini, M.; Bronzato, E.; Tamburin, S.; Bertolasi, L.; Brozzetti, L.; Cecchini, M. P.; Gigli, G.; Monaco, S.; Manganotti, P.; Zanusso, G.

Title:

Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson's disease

Year:

2022

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

TRANSLATIONAL NEURODEGENERATION

ISSN of journal:

2047-9158

N° Volume:

11

Number or Folder:

37

Page numbers:

1-11

Keyword:

Alpha-synuclein; Cerebrospinal fluid; Olfactory mucosa; Parkinson disease; Real-time quaking-induced conversion assay

Short description of contents:

Background: In patients with Parkinson's disease (PD), real-time quaking-induced conversion (RT-QuIC) detection of pathological α-synuclein (α-syn) in olfactory mucosa (OM) is not as accurate as in other α-synucleinopathies. It is unknown whether these variable results might be related to a different distribution of pathological α-syn in OM. Thus, we investigated whether nasal swab (NS) performed in areas with a different coverage by olfactory neuroepithelium, such as agger nasi (AN) and middle turbinate (MT), might affect the detection of pathological α-syn. Methods: NS was performed in 66 patients with PD and 29 non-PD between September 2018 and April 2021. In 43 patients, cerebrospinal fluid (CSF) was also obtained and all samples were analyzed by RT-QuIC for α-syn. Results: In the first round, 72 OM samples were collected by NS, from AN (NSAN) or from MT (NSMT), and 35 resulted positive for α-syn RT-QuIC, including 27/32 (84%) from AN, 5/11 (45%) from MT, and 3/29 (10%) belonging to the non-PD patients. Furthermore, 23 additional PD patients underwent NS at both AN and MT, and RT-QuIC revealed α-syn positive in 18/23 (78%) NSAN samples and in 10/23 (44%) NSMT samples. Immunocytochemistry of NS preparations showed a higher representation of olfactory neural cells in NSAN compared to NSMT. We also observed α-syn and phospho-α-syn deposits in NS from PD patients but not in controls. Finally, RT-QuIC was positive in 22/24 CSF samples from PD patients (92%) and in 1/19 non-PD. Conclusion: In PD patients, RT-QuIC sensitivity is significantly increased (from 45% to 84%) when NS is performed at AN, indicating that α-syn aggregates are preferentially detected in olfactory areas with higher concentration of olfactory neurons. Although RT-QuIC analysis of CSF showed a higher diagnostic accuracy compared to NS, due to the non-invasiveness, NS might be considered as an ancillary procedure for PD diagnosis.

Note:

Erratum in: Correction: Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson's disease. Bongianni M, Catalan M, Perra D, Fontana E, Janes F, Bertolotti C, Sacchetto L, Capaldi S, Tagliapietra M, Polverino P, Tommasini V, Bellavita G, Kachoie EA, Baruca R, Bernardini A, Valente M, Fiorini M, Bronzato E, Tamburin S, Bertolasi L, Brozzetti L, Cecchini MP, Gigli G, Monaco S, Manganotti P, Zanusso G. Transl Neurodegener. 2022 Aug 12;11(1):38. doi: 10.1186/s40035-022-00312-2. PMID: 35962443

Web page:

https://doi.org/10.1186/s40035-022-00311-3

Product ID:

128086

Handle IRIS:

11562/1072309

Last Modified:

February 23, 2023

Bibliographic citation:

Bongianni, M.; Catalan, M.; Perra, D.; Fontana, E.; Janes, F.; Bertolotti, C.; Sacchetto, L.; Capaldi, S.; Tagliapietra, M.; Polverino, P.; Tommasini, V.; Bellavita, G.; Kachoie, E. A.; Baruca, R.; Bernardini, A.; Valente, M.; Fiorini, M.; Bronzato, E.; Tamburin, S.; Bertolasi, L.; Brozzetti, L.; Cecchini, M. P.; Gigli, G.; Monaco, S.; Manganotti, P.; Zanusso, G., Olfactory swab sampling optimization for α-synuclein aggregate detection in patients with Parkinson's disease «TRANSLATIONAL NEURODEGENERATION» , vol. 11 , n. 37 , 2022 , pp. 1-11

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