Publications

Authors:

D'Incà, Erica; Cazzaniga, Stefano; Foresti, Chiara; Vitulo, Nicola; Bertini, Edoardo; Galli, Mary; Gallavotti, Andrea; Pezzotti, Mario; Battista Tornielli, Giovanni; Zenoni, Sara

Title:

VviNAC33 promotes organ de-greening and represses vegetative growth during the vegetative-to-mature phase transition in grapevine

Year:

2021

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

New Phytologist

ISSN of journal:

0028-646X

N° Volume:

231

Number or Folder:

2

:

Blackwell Publishing

Page numbers:

726-746

Keyword:

DAP-seq; NAC33; de-greening; grapevine; phase transition; transcriptomics; vegetative growth; Fruit; Transcriptome; Gene Expression Regulation, Plant; Plant Leaves

Short description of contents:

Plants undergo several developmental transitions during their life cycle. In grapevine, a perennial woody fruit crop, the transition from vegetative/green-to-mature/woody growth involves transcriptomic reprogramming orchestrated by a small group of genes encoding regulators, but the underlying molecular mechanisms are not fully understood.We investigated the function of the transcriptional regulator VviNAC33 by generating and characterizing transgenic overexpressing grapevine lines and a chimeric repressor, and by exploring its putative targets through a DNA affinity purification sequencing (DAP-seq) approach combined with transcriptomic data.We demonstrated that VviNAC33 induces leaf de-greening, inhibits organ growth and directly activates the expression of STAY-GREEN PROTEIN 1 (SGR1), which is involved in Chl and photosystem degradation, and AUTOPHAGY 8f (ATG8f), which is involved in the maturation of autophagosomes. Furthermore, we show that VviNAC33 directly inhibits AUXIN EFFLUX FACILITATOR PIN1, RopGEF1 and ATP SYNTHASE GAMMA CHAIN 1T (ATPC1), which are involved in photosystem II integrity and activity.Our results show that VviNAC33 plays a major role in terminating photosynthetic activity and organ growth as part of a regulatory network governing the vegetative-to-mature phase transition.

Product ID:

125013

Handle IRIS:

11562/1058223

Last Modified:

February 2, 2025

Bibliographic citation:

D'Incà, Erica; Cazzaniga, Stefano; Foresti, Chiara; Vitulo, Nicola; Bertini, Edoardo; Galli, Mary; Gallavotti, Andrea; Pezzotti, Mario; Battista Tornielli, Giovanni; Zenoni, Sara, VviNAC33 promotes organ de-greening and represses vegetative growth during the vegetative-to-mature phase transition in grapevine «New Phytologist» , vol. 231 , n. 2 , 2021 , pp. 726-746

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