Publications

Authors:

Busatto, N.; Farneti, B.; Delledonne, M.; Vrhovsek, U.; Spinelli, F.; Biasioli, F.; Velasco, R.; Costa, G.; Costa, F.

Title:

A multidisciplinary approach reveals new aspects of superficial scald aetiology and cold resistance mechanism in ?Granny Smith? apples

Year:

2019

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Acta horticulturae

ISSN of journal:

0567-7572

N° Volume:

1242

Number or Folder:

1242

Page numbers:

447-454

Keyword:

melo

Short description of contents:

To decipher the transcriptomic regulation of the on-tree fruit maturation in pear cv. ‘Abate Fetel’, a RNA-seq transcription analysis identified 8939 genes differentially expressed across four harvesting stages. These genes were grouped into 11 SOTA clusters based on their transcriptional pattern, of which three included genes upregulated while the other four were represented by downregulated genes. Fruit ripening was furthermore investigated after 1 month of postharvest cold storage. The most important variation in fruit firmness, production of ethylene and volatile organic compounds were observed after 5 days of shelf-life at room temperature following cold storage. The role of ethylene in controlling the ripening of ‘Abate Fetel’ pears was furthermore investigated through the application of 1-methylcyclopropene, which efficiently delayed the progression of ripening by reducing fruit softening and repressing both ethylene and volatile production. The physiological response of the interference at the ethylene receptor level was moreover unraveled investigating the expression pattern of 12 candidate genes, initially selected to validate the RNA-seq profile. This analysis confirmed the effective role of the ethylene competitor in downregulating the expression of cell wall (PG) and ethylene-related genes (ACS, ACO, ERS1, and ERS2), as well as inducing one element involved in the auxin signaling pathway (Aux/IAA), highlighting a possible cross-talk between these two hormones. The expression patterns of these six elements suggest their use as molecular toolkit to monitor at molecular level the progression of the fruit on-tree maturation and postharvest ripening.

Product ID:

124840

Handle IRIS:

11562/1057467

Last Modified:

November 15, 2022

Bibliographic citation:

Busatto, N.; Farneti, B.; Delledonne, M.; Vrhovsek, U.; Spinelli, F.; Biasioli, F.; Velasco, R.; Costa, G.; Costa, F., A multidisciplinary approach reveals new aspects of superficial scald aetiology and cold resistance mechanism in ?Granny Smith? apples «Acta horticulturae» , vol. 1242 , n. 1242 , 2019 , pp. 447-454

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo