Publications

Transcriptional profiling of three Pseudomonas syringae pv. actinidiae biovars reveals different responses to apoplast-like conditions related to strain virulence on host  (2021)

Authors:
Vandelle, Elodie; Colombo, Teresa; Regaiolo, Alice; Maurizio, Vanessa; Libardi, Tommaso; Puttilli, Maria Rita; Danzi, Davide; Polverari, Annalisa
Title:
Transcriptional profiling of three Pseudomonas syringae pv. actinidiae biovars reveals different responses to apoplast-like conditions related to strain virulence on host
Year:
2021
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Referee:
No
Name of journal:
MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN of journal:
0894-0282
Page numbers:
1-59
Keyword:
pseudomonas syringae; bacterial virulence; type III secretion system; c-di-GMP
Short description of contents:
Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen that causes devastating bacterial canker in kiwifruit. Among five biovars defined by genetic, biochemical and virulence traits, Psa3 is the most aggressive and is responsible for the most recent reported outbreaks, but the molecular basis of its heightened virulence is unclear. We therefore designed the first P. syringae multi-strain whole-genome microarray, encompassing biovars Psa1, Psa2 and Psa3 and the well-established model P. syringae pv. tomato, and analyzed early bacterial responses to an apoplast-like minimal medium. Transcriptomic profiling revealed (i) the strong activation in Psa3 of all hrp/hrc cluster genes, encoding components of the type III secretion system required for bacterial pathogenicity and involved in responses to environmental signals; (ii) potential repression of the hrp/hrc cluster in Psa2; and (iii) activation of flagellum-dependent cell motility and chemotaxis genes in Psa1. The detailed investigation of three gene families encoding upstream regulatory proteins (histidine kinases, their cognate response regulators, and proteins with diguanylate cyclase and/or phosphodiesterase domains) indicated that c-di-GMP may be a key regulator of virulence in Psa biovars. The gene expression data were supported by the quantification of biofilm formation. Our findings suggest that diverse early responses to the host apoplast, even among bacteria belonging to the same pathovar, can lead to different virulence strategies and may explain the differing outcomes of infections. Based on our detailed structural analysis of hrp operons, we also propose a revision of hrp cluster organization and operon regulation in P. syringae.
Product ID:
118874
Handle IRIS:
11562/1034126
Last Modified:
February 2, 2025
Bibliographic citation:
Vandelle, Elodie; Colombo, Teresa; Regaiolo, Alice; Maurizio, Vanessa; Libardi, Tommaso; Puttilli, Maria Rita; Danzi, Davide; Polverari, Annalisa, Transcriptional profiling of three Pseudomonas syringae pv. actinidiae biovars reveals different responses to apoplast-like conditions related to strain virulence on host «MOLECULAR PLANT-MICROBE INTERACTIONS»2021pp. 1-59

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo IRIS

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