Publications

Authors:

Balzan, S; Carraro, L; Merlanti, R; Lucatello, L; Capolongo, F; Fontana, F; Novelli, E; Larini, I; Vitulo, N; Cardazzo, B

Title:

Microbial metabarcoding highlights different bacterial and fungal populations in honey samples from local beekeepers and market in north-eastern Italy

Year:

2020

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

International Journal of Food Microbiology

ISSN of journal:

0168-1605

N° Volume:

334

:

Elsevier

Page numbers:

108806-108813

Keyword:

Honey; Microbiota; rRNA 16S; ITS2; Lactic acid bacteria; Bacteria; DNA Barcoding, Taxonomic; DNA, Bacterial; DNA, Fungal; Food Microbiology; Fungi; Honey; Italy; Lactobacillales; Microbiota

Short description of contents:

Due to its chemical properties, honey does not foster the growth of microorganisms, however it may contain a rich microbial community, including viable, stressed, and not viable microbes. In order to characterize honey microbiota focusing on the difference between products from beekeepers and large retail in the present study a culture-independent approach based on DNA metabarcoding was applied. Honey samples were collected from Local Beekeepers (LB) and Market sales (M) during four years with the aim to investigate the microbiological quality in the honey market. Extraction and amplification of DNA from honey samples showed reduced efficiency with increasing age of honey, with the loss of 50-80% of samples four years old (2014). For this reason, only samples of similar age were compared and the analysis of microbial communities focused on year 2017, for a total of 75 samples. Differences in alpha and beta-diversity were evidenced comparing microbial communities between LB and M samples. In particular, contaminant bacteria dominated the microbiota in M samples while LB samples were enriched in Lactic Acid Bacteria (LAB) that cannot be isolated with culture-dependent approaches.

Product ID:

117509

Handle IRIS:

11562/1029747

Last Modified:

November 15, 2022

Bibliographic citation:

Balzan, S; Carraro, L; Merlanti, R; Lucatello, L; Capolongo, F; Fontana, F; Novelli, E; Larini, I; Vitulo, N; Cardazzo, B, Microbial metabarcoding highlights different bacterial and fungal populations in honey samples from local beekeepers and market in north-eastern Italy «International Journal of Food Microbiology» , vol. 334 , 2020 , pp. 108806-108813

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