Genomica e trascrittomica (2012/2013)

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Course code
4S02850
Name of lecturer
Massimo Delledonne
Coordinator
Massimo Delledonne
Number of ECTS credits allocated
6
Other available courses
Academic sector
BIO/18 - GENETICS
Language of instruction
Italian
Location
VERONA
Period
I semestre dal Oct 1, 2012 al Jan 31, 2013.
Course news Seminars related to the course

Lesson timetable

Learning outcomes

OBJECTIVES
The course structure and function of genomes aims to provide the student with knowledge about the structure of eukaryotic and prokaryotic genomes, and methods of global analysis of genomes and transcriptomes. It also provides information about the replication and evolution of genomes, with particular emphasis on mecchanisms that regulate this activity.


CONTENTS
The genome projects: organization and goals of the Human Genome Project, plants and animals genomes. Mapping, sequencing and assembly of genomes. Interpretation of genomic sequences and global analysis of gene expression. Anatomy of eukaryotic and prokaryotic genomes. Operation of genomes and regulation of their activities. Replication and evolution of genomes. molecular phylogenesis

Syllabus

1- Genome mapping (dispense)
Genetic and physical maps
Molecular markers
Gene tagging
Tail PCR
AIMS

2 - Trancriptome analysis (dispense)
cDNA
Northern blot
RT-PCR
Real Time RT PCR
cDNA libraries
RACE (rapid amplification of cDNA ends)
Subtractive libraries
Reverse Northern
Differential display / CDNA-AFLP
SAGE (Serial Analysis of Gene Expression)
EST and electronic northern
Microarray
RNA-Seq

3 - Epigenetics (articoli)
DNA methilation
Histone modifications
Methods to analyze methilated DNA

4 - Genome analysis (articoli)
DNA sequencing methods
Genome assembly
Localization of genes in a genomic sequence
Functional characterization of genes (in silico and in vivo)
Forward and Reverse genetics
Genome wide association studies
Personal genomics (molecular diagnostics)

5 - Structure, function and evolution of plant and animal genomes (articoli)
The Arabidopsis geneome and the 1001 genomes project
The human genome (IHGC, Celera) and the 1000 genomes project
The “minimal genome” project
The ENCODE project
Human genome interpretation(Ingenuity, Knome etc.)


Suggested Text books: Genomi 3 EdiSES ISBN 978 88 7959 431 8
Supplemental material is available here: http://profs.sci.univr.it/delledonne/Insegnamenti/Index.html

Assessment methods and criteria

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