Enzymology - mod.1 (2008/2009)

Learning outcomes

Basis for the comprehension of enzyme catalysis (kinetc equations, mechanisms of catalysis, factors that influences the stability and activity of enzymes): Methods for enzyme engineering (rational design, modification of selectivity, stability, ntroduction of elements for the regulation of enzyme activity)

Introduction. Enzyme classification. Structural features of enzymes.
Enzyme kinetic: prestationary and stationary phase; kinetic equaitions, kinetic parameters, linearization, kinetic equation for ordered bi bi mechanism, kinetic equation for ping pong bi bi mechanism, secondary graphs, inhibition and activation and relative kinetic models.
Mechanism of catalysis: transition state teory, catalysis acid/base, nucleophiles catalysis, electrophyl catalysis, covalent catalysis.
Activity and stability of enzymes. Optimization of stability.
Selection of estremophyles, mutagenesis, immobilization of enzymes.
Engineering of enzyme selectivity: situ specific mutagenesis, rational design, random mutagenesis.
Enzymes in organic solvent.
Synthetic biocatalysts.
Regulation of catalytic activity, allosteric sites, molecular switches.

Oral Bioinformatica
Written (2 hours) LS Biotec Molecolare ed Industriale