Molecular biology of micro-organisms (2008/2009)

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Course code
Name of lecturer
Massimiliano Perduca
Number of ECTS credits allocated
Academic sector
Language of instruction
2° Sem dal Mar 9, 2009 al Jun 12, 2009.

Lesson timetable

Learning outcomes

The aim of this course is to give the students, already proficient in the fundamentals acquired during the General Molecular Biology and General and Applied Microbiology course, deeper elements concerning the central topics of Molecular biology, with emphasis on microorganisms.


-> RNAs and transcription
Different types of RNA: synthesis and maturation. Bacterial RNA polymerase. Sigma factors. Eukaryotic RNA polymerases. Eukaryotic mRNAs: capping, polyadenylation, cytoplasmic localization. The transcription process in bacteria and in eukaryotic cells.
-> Regulation of gene expression 2
Bacterial promoters. The operon. Activators, repressors and coactivators. Signal transductions and two component regulation systems.
-> Translation
Ribosomes. tRNA structure and function. Aminoacyl-tRNA synthesis. Initiation in bacteria and eukaryotic cells. Polypeptide chain synthesis and translation end. Regulation of translation. Protein localization.
-> Viruses: fundamental elements
Virus general properties. Structure of viral particles. Virus growing. Test and count methods for viruses. General features of viral reproduction. Baterial viruses or bacteriophages: a) RNA phages ; b) single stranded DNA phages; c) double stranded DNA phages; d) tempered bacteriophages and lysogeny (Lambda phage); e) transposable phages (Mu bacteriophage).
-> Transposable elements 2
Retroviruses and retrotransposones.
-> Microbial genetics
Transformation. Transduction. Plasmids. Conjugation.

Reference books
Author Title Publisher Year ISBN Note
WATSON James D., BAKER Tania A., BELL Stephen P., GANN Alexander, LEVINE Michael, LOSICK Richard Biologia molecolare del gene (Edizione 5) Zanichelli 2005 978-8808-17890-9
MADIGAN Michael T., MARTINKO John M. Brock Bilogia dei Microrganismi Volume 1 Microbiologia Generale Ambrosiana 2007 978-88-408-1375-2
ALLISON Lizabeth A. Fondamenti di Biologia Molecolare Zanichelli 2008 978-8808-16622-7
LEWIN Benjamin IL GENE Edizione compatta Zanichelli 2006 978-8808-06877-4
LEWIN Benjamin Il Gene VIII Zanichelli 2006 978-8808-17902-9
Harvey Lodish, Chris A. Kaiser, Anthony Bretscher, Angelika Amon, Arnold Berk, Monty Krieger, Hidde Ploegh and Matthew P. Scott Molecular Cell Biology (Edizione 7) Freeman 2012 1464102325

Assessment methods and criteria

Oral examination