Chemistry and Analytical Biochemistry - Biochimica analitica - teoria (2006/2007)

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Course code
Name of lecturer
Massimiliano Perduca
Number of ECTS credits allocated
Academic sector
Language of instruction
2nd semester dal Mar 1, 2007 al Jun 8, 2007.

To show the organization of the course that includes this module, follow this link * Course organization

Lesson timetable

2nd semester
Day Time Type Place Note
Monday 10:30 AM - 12:30 PM lesson Lecture Hall I from Apr 2, 2007  to Jun 8, 2007
Wednesday 8:30 AM - 10:30 AM lesson Lecture Hall I from Apr 2, 2007  to Jun 8, 2007
Friday 8:30 AM - 10:30 AM lesson Lecture Hall H from Apr 2, 2007  to Jun 8, 2007

Learning outcomes

Fundamentals of applied methodology for protein analysis.


Protein purification. Cell rupture. Protein solubilisation methods.
Colorimetric and spectroscopic methods for protein quantification.
Spectroscopic and spectrofluorimetric methods for tertiary and quaternaly structure analysis.

Chromatographic methods for protein purification. Ionic exchange, size exclusion, affiity, methal chelate, covalent.

Electrophoresis for protein purification. Polyacrylamide gel. SDS electrohoresis. Porosity gradient gel. Isoelectrofocusing. Two-diemnsional electrophoresis. Western blotting. Staining of gels. Gel electrophoresis of DNA. CApillary electrophoresis of proteins, peptides and DNA.

Reference books
Author Title Publisher Year ISBN Note
J. Walker, K. Wilson Metodologia biochimica. Le bioscienze e le biotecnologie in laboratorio. (Edizione 5) Raffaello Cortina Editore 2001 8870786870 Presenti due copie nella biblioteca di Facoltà

Assessment methods and criteria

Oral exam