Pubblicazioni

Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study  (2016)

Autori:
Salvetti, Elisa; Orrù, Luigi; Capozzi, Vittorio; Martina, Alessia; Lamontanara, Antonella; Keller, David; Cash, Howard; Felis, Giovanna; Cattivelli, Luigi; Torriani, Sandra; Spano, Giuseppe
Titolo:
Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study
Anno:
2016
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN Rivista:
0175-7598
N° Volume:
100
Numero o Fascicolo:
10
Intervallo pagine:
4595-4605
Parole chiave:
Antibiotic resistance; Bacillus coagulans; Genomics; Probiotics; Safety; Virulence factors
Breve descrizione dei contenuti:
Probiotics are microorganisms that confer beneficial effects on the host; nevertheless, before being allowed for human consumption, their safety must be verified with accurate protocols. In the genomic era, such procedures should take into account the genomic-based approaches. This study aims at assessing the safety traits of Bacillus coagulans GBI-30, 6086 integrating the most updated genomics-based procedures and conventional phenotypic assays. Special attention was paid to putative virulence factors (VF), antibiotic resistance (AR) genes and genes encoding enzymes responsible for harmful metabolites (i.e. biogenic amines, BAs). This probiotic strain was phenotypically resistant to streptomycin and kanamycin, although the genome analysis suggested that the AR-related genes were not easily transferrable to other bacteria, and no other genes with potential safety risks, such as those related to VF or BA production, were retrieved. Furthermore, no unstable elements that could potentially lead to genomic rearrangements were detected. Moreover, a workflow is proposed to allow the proper taxonomic identification of a microbial strain and the accurate evaluation of risk-related gene traits, combining whole genome sequencing analysis with updated bioinformatics tools and standard phenotypic assays. The workflow presented can be generalized as a guideline for the safety investigation of novel probiotic strains to help stakeholders (from scientists to manufacturers and consumers) to meet regulatory requirements and avoid misleading information.
Id prodotto:
95186
Handle IRIS:
11562/955411
ultima modifica:
4 dicembre 2019
Citazione bibliografica:
Salvetti, Elisa; Orrù, Luigi; Capozzi, Vittorio; Martina, Alessia; Lamontanara, Antonella; Keller, David; Cash, Howard; Felis, Giovanna; Cattivelli, Luigi; Torriani, Sandra; Spano, Giuseppe, Integrate genome-based assessment of safety for probiotic strains: Bacillus coagulans GBI-30, 6086 as a case study «APPLIED MICROBIOLOGY AND BIOTECHNOLOGY» , vol. 100 , n. 102016pp. 4595-4605

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Titolo Dipartimento Responsabili
Realizzazione di una innovativa pasta alimentare funzionale arricchita di componenti bioattivi e probiotic (PASS-WORLD –Pasta e salute nel mondo) Dipartimento Biotecnologie Sandra Torriani
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