Nuclear magnetic resonance and imaging (2010/2011)

Course not running

Course code
4S000659
Credits
6
Coordinator
Henriette Molinari
Academic sector
CHIM/06 - ORGANIC CHEMISTRY
Language of instruction
Italian
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
Teoria 5 I semestre Henriette Molinari
Laboratorio 1 I semestre Henriette Molinari

Lesson timetable

I semestre
Activity Day Time Type Place Note
Teoria Tuesday 9:30 AM - 11:30 AM lesson Lecture Hall L  
Teoria Wednesday 11:30 AM - 1:30 PM lesson Lecture Hall L  
Laboratorio Thursday 11:30 AM - 1:30 PM lesson Laboratory Gamma  

Learning outcomes

The aim of the course is to provide the students with the knowledge of one of the most important methodology in the field of life science, namely Nuclear Magnetic Resonance (NMR) and its application to molecular Imaging. The course will deal with the basic proinciples of NMR and its applications aimed at the investigation, at molecular level, of: i) structure-function correlation of biological macromolecules, ii) protein-protein, protein-small molecule, protein-membrane interactions, iii) mechanism of protein folding, iv) molecular dynamics, v) complex systems “in cell”, vi) in vitro and in vivo imaging techniques

Syllabus

Basic principles of NMR
The NMR spectrometer
Radiofrequency pulses
Molecular motion and relaxation. T1 and T2
NMR parameters: Chemical shift, J coupling, dipolar coupling, NOE
One dimensional NMR
Two-dimensional and multi-dimensional NMR experiments
Sequential assignments of proteins
Structural calculations and structure validation
Protein-ligand, protein-protein, protein membrane interactions via NMR
KD determination
In cell NMR
Basis of Imaging: Introduction, gradients, spatial encoding of the resonance, Image formation, basic pulse schemes, spin-echo, image contrast and weighting (T1, T2, T2* proton density).

Assessment methods and criteria

The examination will deal with the presentation of a paper, suggested by the teacher, and related to the issues treated during the course.
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