Gene design and DNA arrays laboratory (2009/2010)

Course partially running

Course code
4S00202
Credits
3
Coordinator
Tiziana Pandolfini
Teaching is organised as follows:
Unit Credits Academic sector Period Academic staff
GENE DESIGN 1 BIO/04-PLANT PHYSIOLOGY 1st Semester Tiziana Pandolfini
LABORATORIO DI DNA ARRAYS 2 BIO/04-PLANT PHYSIOLOGY 1st Semester Tiziana Pandolfini

Learning outcomes

Module: LABORATORIO DI DNA ARRAYS
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The objective is to learn how to perform a DNA-microarray experiment of gene expression analysis.


Module: GENE DESIGN
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The objective of the course is the acquisition of knowledge about modern methods of transcriptome analysis and methodological approaches to functional genomics. The course examines DNA array technology describing how microarrays are manufactured and used.

Syllabus

Module: LABORATORIO DI DNA ARRAYS
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Extraction and purification of total RNA and mRNA from different tissues. RNA amplification and cDNA production. Direct and indirect cDNA labelling. Microarray hybridisation and image acquisition.

Module: GENE DESIGN
Design of gene constructs for functional analysis of genes.
Methods of transcriptome analysis. DNA microarrays: experimental design, cDNA and oligonucleotide microarrays. Microarray printing. Total RNA and mRNA extraction, RNA amplification. cDNA synthesis and labeling. Microarray hybridisation and data acquisition. Applications of microarray technology.

Assessment methods and criteria

Module: LABORATORIO DI DNA ARRAYS
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oral


Module: GENE DESIGN
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oral

Studying