Pubblicazioni

Autori:

Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola; Forcato, Claudio; D'Angelo, Michela; Schiavon, Riccardo; Vezzi, Alessandro; Giacometti, Giorgio Mario; Morosinotto, Tomas; Valle, Giorgio

Titolo:

Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion

Anno:

2014

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

No

Nome rivista:

MOLECULAR PLANT

ISSN Rivista:

1674-2052

N° Volume:

7

Numero o Fascicolo:

2

Intervallo pagine:

323-335

Parole chiave:

Nannochloropsis; SOLiD; biofuel; chromosomes; de novo assembly; genome; lipids; nitrogen deprivation; telomeres.; transcriptome; Chromosomes; Gene Expression Regulation; Molecular Sequence Annotation; Nitrogen; Stramenopiles; Gene Expression Profiling; Genome

Breve descrizione dei contenuti:

Nannochloropsis is rapidly emerging as a model organism for the study of biofuel production in microalgae. Here, we report a high-quality genomic assembly of Nannochloropsis gaditana, consisting of large contigs, up to 500 kbp long, and scaffolds that in most cases span the entire length of the chromosomes. We identified 10646 complete genes and characterized possible alternative transcripts. The annotation of the predicted genes and the analysis of cellular processes revealed traits relevant for the genetic improvement of this organism such as genes involved in DNA recombination, RNA silencing, and cell wall synthesis. We also analyzed the modification of the transcriptional profile in nitrogen deficiency-a condition known to stimulate lipid accumulation. While the content of lipids increased, we did not detect major changes in expression of the genes involved in their biosynthesis. At the same time, we observed a very significant down-regulation of mitochondrial gene expression, suggesting that part of the Acetyl-CoA and NAD(P)H, normally oxidized through the mitochondrial respiration, would be made available for fatty acids synthesis, increasing the flux through the lipid biosynthetic pathway. Finally, we released an information resource of the genomic data of N. gaditana, available online at www.nannochloropsis.org.

Id prodotto:

97719

Handle IRIS:

11562/963626

ultima modifica:

16 novembre 2022

Citazione bibliografica:

Corteggiani Carpinelli, Elisa; Telatin, Andrea; Vitulo, Nicola; Forcato, Claudio; D'Angelo, Michela; Schiavon, Riccardo; Vezzi, Alessandro; Giacometti, Giorgio Mario; Morosinotto, Tomas; Valle, Giorgio, Chromosome scale genome assembly and transcriptome profiling of Nannochloropsis gaditana in nitrogen depletion «MOLECULAR PLANT» , vol. 7 , n. 2 , 2014 , pp. 323-335

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo