Pubblicazioni

Secretome protein signature of human pancreatic cancer stem-like cells  (2016)

Autori:
Brandi, Jessica; Dalla Pozza, Elisa; Dando, Ilaria; Biondani, Giulia; Robotti, Elisa; Jenkins, Rosalind; Elliott, Victoria; Park, Kevin; Marengo, Emilio; Costello, Eithne; Scarpa, Aldo; Palmieri, Marta; Cecconi, Daniela
Titolo:
Secretome protein signature of human pancreatic cancer stem-like cells
Anno:
2016
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
JOURNAL OF PROTEOMICS
ISSN Rivista:
1874-3919
N° Volume:
136
Intervallo pagine:
1-12
Parole chiave:
Pancreatic cancer; Secretome; stem-cell
Breve descrizione dei contenuti:
Emerging research has demonstrated that pancreatic ductal adenocarcinoma (PDAC) contains a sub-population of cancer stem cells (CSCs) characterized by self-renewal, anchorage-independent-growth, long-term proliferation and chemoresistance. The secretome analysis of pancreatic CSCs has not yet been performed, although it may provide insight into tumour/microenvironment interactions and intracellular processes, as well as to identify potential biomarkers. To characterize the secreted proteins of pancreatic CSCs, we performed an iTRAQ-based proteomic analysis to compare the secretomes of Panc-1 cancer stem-like cells (Panc1 CSCs) and parental cell line. A total of 72 proteins were found up-/down-regulated in the conditioned medium of Panc-1 CSCs. The pathway analysis revealed modulation of vital physiological pathways including glycolysis, gluconeogenesis and pentose phosphate. Through ELISA immunoassays we analysed the presence of the three proteins most highly secreted by Panc-1 CSCs (ceruloplasmin, galectin-3, and MARCKS) in sera of PDAC patient. ROC curve analysis suggests ceruloplasmin as promising marker for patients negative for CA19-9. Overall, our study provides a systemic secretome analysis of pancreatic CSCs revealing a number of secreted proteins which participate in pathological conditions including cancer differentiation, invasion and metastasis. They may serve as a valuable pool of proteins from which biomarkers and therapeutic targets can be identified.
Pagina Web:
http://www.sciencedirect.com/science/article/pii/S1874391916300203
Id prodotto:
90646
Handle IRIS:
11562/935533
ultima modifica:
27 settembre 2022
Citazione bibliografica:
Brandi, Jessica; Dalla Pozza, Elisa; Dando, Ilaria; Biondani, Giulia; Robotti, Elisa; Jenkins, Rosalind; Elliott, Victoria; Park, Kevin; Marengo, Emilio; Costello, Eithne; Scarpa, Aldo; Palmieri, Marta; Cecconi, Daniela, Secretome protein signature of human pancreatic cancer stem-like cells «JOURNAL OF PROTEOMICS» , vol. 1362016pp. 1-12

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo IRIS

Progetti Collegati
Titolo Dipartimento Responsabili
Ricerca di marcatori molecolari proteici nel tumore pancreatico tramite analisi proteomiche di cellule staminali tumorali Dipartimento Biotecnologie Daniela Cecconi, Marta Palmieri
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