Pubblicazioni

Autori:

De Souza Lima Oliveira, Gabriel; Salvagno, GIAN LUCA; Danese, Elisa; Favaloro, Ej; Guidi, Giancesare; Lippi, Giuseppe

Titolo:

Sodium citrate blood contamination by K2 -ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing.

Anno:

2015

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY

ISSN Rivista:

1751-5521

N° Volume:

37

Numero o Fascicolo:

3

Intervallo pagine:

403-409

Parole chiave:

EDTA; K(3)EDTA vacuum tubes; Contamination

Breve descrizione dei contenuti:

INTRODUCTION: The potential cross-contamination of additives between primary blood tubes is a well-known problem during sample collection. The aim of this study was to assess the impact of citrated blood contamination with different amounts of dipotassium ethylenediaminetetraacetic (K2 EDTA blood) on activated partial thromboplastin time (APTT), prothrombin time (PT), and fibrinogen. METHODS: Blood was collected from 15 ostensibly healthy volunteers into four 0.109 m citrate blood tubes followed by one K2 EDTA blood tube. The citrate tubes of each subject were pooled and divided in five aliquots. The whole blood of the K2 EDTA tube was then added in scalar amounts to autologous citrated blood aliquots, to obtain K2 EDTA contamination ranging from 0% to 43%, and thus mimic potential pre-analytical contamination. RESULTS: A statistically and clinically significant prolongation was observed for both APTT and PT between 29% and 43% K2 EDTA contamination, whereas the decrease of fibrinogen values became statistically and clinically significant at 43% K2 EDTA contamination. CONCLUSION: The results of this investigation show that contamination of citrated blood with as much as 29% of K2 EDTA blood generates a significant bias in results of routine clotting assays. This has serious implications for patient safety and management.

Id prodotto:

86956

Handle IRIS:

11562/918391

depositato il:

7 maggio 2015

ultima modifica:

15 novembre 2022

Citazione bibliografica:

De Souza Lima Oliveira, Gabriel; Salvagno, GIAN LUCA; Danese, Elisa; Favaloro, Ej; Guidi, Giancesare; Lippi, Giuseppe, Sodium citrate blood contamination by K2 -ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing. «INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY» , vol. 37 , n. 3 , 2015 , pp. 403-409

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