Pubblicazioni

Autori:

Astone, Matteo; Tesoriero, Chiara; Schiavone, Marco; Facchinello, Nicola; Tiso, Natascia; Argenton, Francesco; Vettori, Andrea

Titolo:

Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish

Anno:

2024

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN Rivista:

1422-0067

N° Volume:

25

Numero o Fascicolo:

18

Intervallo pagine:

1-20

Parole chiave:

Wnt/beta-catenin; Yap/Taz; Hippo; zebrafish; crosstalk; embryonic development

Breve descrizione dei contenuti:

Hippo-YAP/TAZ and Wnt/beta-catenin signaling pathways, by controlling proliferation, migration, cell fate, stemness, and apoptosis, are crucial regulators of development and tissue homeostasis. We employed zebrafish embryos as a model system to elucidate in living reporter organisms the crosstalk between the two signaling pathways. Co-expression analysis between the Wnt/beta-catenin Tg(7xTCF-Xla.Siam:GFP)ia4 and the Hippo-Yap/Taz Tg(Hsa.CTGF:nlsmCherry)ia49 zebrafish reporter lines revealed shared spatiotemporal expression profiles. These patterns were particularly evident in key developmental regions such as the midbrain-hindbrain boundary (MHB), epidermis, muscles, neural tube, notochord, floorplate, and otic vesicle. To investigate the relationship between the Wnt/beta-catenin pathway and Hippo-Yap/Taz signaling in vivo, we conducted a series of experiments employing both pharmacological and genetic strategies. Modulation of the Wnt/beta-catenin pathway with IWR-1, XAV939, or BIO resulted in a significant regulation of the Yap/Taz reporter signal, highlighting a clear correlation between beta-catenin and Yap/Taz activities. Furthermore, genetic perturbation of the Wnt/beta-catenin pathway, by APC inhibition or DKK1 upregulation, elicited evident and robust alteration of Yap/Taz activity. These findings revealed the intricate regulatory mechanisms underlying the crosstalk between the Wnt/beta-catenin and Hippo-Yap/Taz signaling, shedding light on their roles in orchestrating developmental processes in vivo.

Pagina Web:

https://doi.org/10.3390/ijms251810005

Id prodotto:

141893

Handle IRIS:

11562/1143486

ultima modifica:

6 novembre 2024

Citazione bibliografica:

Astone, Matteo; Tesoriero, Chiara; Schiavone, Marco; Facchinello, Nicola; Tiso, Natascia; Argenton, Francesco; Vettori, Andrea, Wnt/β-Catenin Signaling Regulates Yap/Taz Activity during Embryonic Development in Zebrafish «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 25 , n. 18 , 2024 , pp. 1-20

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