Pubblicazioni

Autori:

Molesini, Barbara; Pennisi, Federica; Vitulo, Nicola; Pandolfini, Tiziana

Titolo:

MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Referee:

No

Nome rivista:

BMC RESEARCH NOTES

ISSN Rivista:

1756-0500

N° Volume:

16

Numero o Fascicolo:

242

Intervallo pagine:

1-6

Parole chiave:

Fruit set; MicroRNA; Parthenocarpy; RNA silencing; RNA-Seq; Tomato

Breve descrizione dei contenuti:

Fruit set is triggered after ovule fertilization, as a consequence of the downregulation of ovary growth repressors, such as the tomato transcription factors Auxin/indole-3-acetic acid 9 (IAA9) and Agamous-like 6 (AGL6). In a recent work, we developed a method to silence IAA9 and AGL6 in tomato ovaries using exogenous dsRNAs. We also produced small RNA libraries from IAA9- and AGL6-silenced ovaries to confirm the presence of siRNAs, derived from exogenous dsRNA, targeting IAA9 and AGL6. The objective of this work is to exploit these sRNA libraries to identify miRNAs differentially expressed in IAA9- and AGL6-silenced ovaries as compared with unpollinated control ovaries.ResultsWe identified by RNA sequencing 125 and 104 known and 509 and 516 novel miRNAs from reads mapped to mature or hairpin sequences, respectively. Of the known miRNAs, 7 and 45 were differentially expressed in IAA9- and AGL6-silenced ovaries compared to control ones, respectively. Six miRNAs were common to both datasets, suggesting their importance in the fruit set process. The expression pattern of two of these (miR393 and miR482e-5p) was verified by stem-loop qRT-PCR. The identified miRNAs represent a pool of regulatory sRNAs potentially involved in tomato fruit initiation.

Id prodotto:

136762

Handle IRIS:

11562/1116057

ultima modifica:

5 febbraio 2025

Citazione bibliografica:

Molesini, Barbara; Pennisi, Federica; Vitulo, Nicola; Pandolfini, Tiziana, MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set «BMC RESEARCH NOTES» , vol. 16 , n. 242 , 2023 , pp. 1-6

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