Pubblicazioni

Autori:

Parmagnani, Ambra S.; Giuseppe, Mannino; Carla, Brillada; Mara, Novero; Dall'Osto, Luca; Maffei, Massimo E.

Titolo:

Biology of Two-Spotted Spider Mite (Tetranychus urticae): Ultrastructure, Photosynthesis, Guanine Transcriptomics, Carotenoids and Chlorophylls Metabolism, and Decoyinine as a Potential Acaricide

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN Rivista:

1422-0067

N° Volume:

24

Numero o Fascicolo:

1715

Intervallo pagine:

1-22

Parole chiave:

Phaseolus lunatus

Breve descrizione dei contenuti:

: Two-Spotted Spider Mites (TSSMs, Tetranychus urticae Koch 1836 (Acari: Tetranychidae)) is one of the most important pests in many crop plants, and their feeding activity is based on sucking leaf cell contents. The purpose of this study was to evaluate the interaction between TSSMs and their host Lima bean (Phaseolus lunatus) by analyzing the metabolomics of leaf pigments and the transcriptomics of TSSM guanine production. We also used epifluorescence, confocal laser scanning, and transmission electron microscopies to study the morphology and structure of TSSMs and their excreta. Finally, we evaluated the potential photosynthetic ability of TSSMs and the activity and content of Ribulose-1,5-bisphosphate Carboxylase/Oxigenase (RubisCO). We found that TSSMs express several genes involved in guanine production, including Guanosine Monophosphate Synthetase (GMPS) and decoyinine (DCY), a potential inhibitor of GMPS, was found to reduce TSSMs proliferation in infested Lima bean leaves. Despite the presence of intact chloroplasts and chlorophyll in TSSMs, we demonstrate that TSSMs do not retain any photosynthetic activity. Our results show for the first time the transcriptomics of guanine production in TSSMs and provide new insight into the catabolic activity of TSSMs on leaf chlorophyll and carotenoids. Finally, we preliminary demonstrate that DCY has an acaricidal potential against TSSMs.

Id prodotto:

132793

Handle IRIS:

11562/1088266

ultima modifica:

28 marzo 2023

Citazione bibliografica:

Parmagnani, Ambra S.; Giuseppe, Mannino; Carla, Brillada; Mara, Novero; Dall'Osto, Luca; Maffei, Massimo E., Biology of Two-Spotted Spider Mite (Tetranychus urticae): Ultrastructure, Photosynthesis, Guanine Transcriptomics, Carotenoids and Chlorophylls Metabolism, and Decoyinine as a Potential Acaricide «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 24 , n. 1715 , 2023 , pp. 1-22

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo