Pubblicazioni

Autori:

Peron, Margherita; Dinarello, Alberto; Meneghetti, Giacomo; Martorano, Laura; Facchinello, Nicola; Vettori, Andrea; Licciardello, Giorgio; Tiso, Natascia; Argenton, Francesco

Titolo:

The stem-like Stat3-responsive cells of zebrafish intestine are Wnt/β-catenin dependent

Anno:

2020

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

Development

ISSN Rivista:

0950-1991

N° Volume:

147

Numero o Fascicolo:

12

Intervallo pagine:

1-13

Parole chiave:

Intestine; Stat3; Stem cells; Wnt/β-catenin; Zebrafish

Breve descrizione dei contenuti:

The transcription factor Stat3 is required for proliferation and pluripotency of embryonic stem cells; we have prepared and characterized fluorescent Stat3-reporter zebrafish based on repeats of minimal responsive elements. These transgenic lines mimic in vivo Stat3 expression patterns and are responsive to exogenous Stat3; notably, fluorescence is inhibited by both stat3 knockout and IL6/Jak/STAT inhibitors. At larval stages, Stat3 reporter activity correlates with proliferating regions of the brain, haematopoietic tissue and intestine. In the adult gut, the reporter is active in sparse proliferating cells, located at the base of intestinal folds, expressing the stemness marker sox9b and having the morphology of mammalian crypt base columnar cells; noteworthy, zebrafish stat3 mutants show defects in intestinal folding. Stat3 reporter activity in the gut is abolished with mutation of T cell factor 4 (Tcf7l2), the intestinal mediator of Wnt/β-catenin-dependent transcription. The Wnt/β-catenin dependence of Stat3 activity in the gut is confirmed by abrupt expansion of Stat3-positive cells in intestinal adenomas of apc heterozygotes. Our findings indicate that Jak/Stat3 signalling is needed for intestinal stem cell maintenance and possibly crucial in controlling Wnt/β-catenin-dependent colorectal cancer cell proliferation.

Id prodotto:

115237

Handle IRIS:

11562/1020479

ultima modifica:

24 gennaio 2025

Citazione bibliografica:

Peron, Margherita; Dinarello, Alberto; Meneghetti, Giacomo; Martorano, Laura; Facchinello, Nicola; Vettori, Andrea; Licciardello, Giorgio; Tiso, Natascia; Argenton, Francesco, The stem-like Stat3-responsive cells of zebrafish intestine are Wnt/β-catenin dependent «Development» , vol. 147 , n. 12 , 2020 , pp. 1-13

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo