ANALYTICAL AND CLINICAL PROTEOMICS (2011/2012)

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Course code
4S000680
Credits
6
Coordinator
Daniela Cecconi
Other available courses
Other available courses
    Academic sector
    CHIM/01 - ANALYTICAL CHEMISTRY
    Language of instruction
    Italian
    Teaching is organised as follows:
    Activity Credits Period Academic staff Timetable
    Teoria 5 II semestre Daniela Cecconi
    Laboratorio 1 II semestre Daniela Cecconi

    Lesson timetable

    II semestre
    Activity Day Time Type Place Note
    Teoria Tuesday 8:30 AM - 10:30 AM lesson Lecture Hall H  
    Teoria Thursday 11:30 AM - 1:30 PM lesson Lecture Hall H  

    Learning outcomes

    Module:
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    Clinical proteomics is the science that aims to develop technologies for the treatment of patients through the analysis of proteomes of healthy and sick cells. This research area aimed to identify proteins that can be used as early markers in blood, urine, or tissues of cancer patients, or that may predict response to therapy or the relapse after treatment.

    Syllabus

    Module:
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    Analytical proteomics (LM9+LM7):

    1. Introduction.

    2. Sample preparation.

    3. Gel-based separation.

    4. 2D gel Image analysis.

    5. Gel-free separation.

    6. Pre-fractionation of low abundance proteins.

    7. Analysis of proteins/peptides by Mass Spectrometry.

    8. Analysis of proteins/peptides by Tandem Mass Spectrometry.

    9. Analysis of mass spectra and database search for protein identification.

    10. Differential and Quantitative proteomics.

    11. Characterization of post-translational modifications.

    12. Proteomics in agro-food area.



    Clinical proteomics (LM9):

    13. Cancer (I).

    14. Cancer (II).

    15. Using proteomics to study mechanism of pharmacological action of new drugs

    16. Cardiac Disease.

    17. Vascular Disease and Hypertension.

    18. Hemochromatosis and Intrauterine growth retardation

    19. Bacterial and viral infections.

    20. Autoimmune disease.


    RECOMMENDED TEXT BOOKS:
    “Introducing Proteomics: From concepts to sample separation, mass spectrometry and data analysis” Josip Lovric, Oxford : Wiley-Blackwell, (textbook) (LM7, LM9)

    “Clinical Proteomics: From Diagnosis to Therapy” Jennifer E. Van Eyk (Editor), Michael J. Dunn (Editor) Wiley & Sons Ltd, ISBN: 978-3-527-31637-3 Hardcover (LM9)

    Assessment methods and criteria

    Module:
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    LM7: written
    LM9: mini oral presentation in group + written