| Unit | Credits | Academic sector | Period | Academic staff |
|---|---|---|---|---|
| Laboratorio di DNA arrays | 2 | BIO/04-PLANT PHYSIOLOGY | 1° Sem |
Tiziana Pandolfini
|
| Gene design | 1 | BIO/04-PLANT PHYSIOLOGY | 1° Sem |
Tiziana Pandolfini
|
Module: Gene design
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The objective of the course is the acquisition of knowledge about modern methods of transcriptome analysis and methodological approaches to functional genomics. The course examines DNA array technology describing how microarrays are manufactured and used.
Module: Laboratorio di DNA arrays
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The objective is to learn how to perform a DNA-microarray experiment of gene expression analysis.
Module: Gene design
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Methods of transcriptome analysis. DNA microarrays: experimental design, cDNA and oligonucleotide microarrays. Microarray printing. Total RNA and mRNA extraction, RNA amplification. cDNA synthesis and labeling. Microarray hybridisation and data acquisition. Applications of microarray technology. Design of gene constructs for functional analysis of genes.
Module: Laboratorio di DNA arrays
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Extraction and purification of total RNA and mRNA from different tissues. RNA amplification and cDNA production. Direct and indirect cDNA labelling. Microarray hybridisation and image acquisition.
Module: Gene design
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oral
Module: Laboratorio di DNA arrays
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oral
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