| Unit | Credits | Academic sector | Period | Academic staff |
|---|---|---|---|---|
| Teoria | 5 | BIO/11-MOLECULAR BIOLOGY | 2° Sem |
Ugo Luigi Monaco
|
| Laboratorio | 1 | BIO/11-MOLECULAR BIOLOGY | 2° Sem |
Massimiliano Perduca
|
Module: Teoria
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Module: Laboratorio
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The Biocrystallography laboratory introduces the students into the fundamentals of the field, particularly biological crystal growth. In addition to coveringh the practical aspects of protein crystal preparation, a diffraction data is collected and a protein structure is solved.
Module: Teoria
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Module: Laboratorio
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The Biocrystallography practical lessons entail a series of meetings focused on the following arguments:
1) An introduction covering macromolecular crystallization theory and the most used techniques.
2) Preparation of protein crystals using Microdialysis, Hanging and Sitting drop.
3) Collection of a dataset with a rotating anode conventional source, an image plate detector and a low temperature cooling system.
4) Data processing and analysis.
5) Solution of a protein structure using molecular replacement techniques.
6) Protein model building and refinement.
7) Presentation of the most widely used programs for molecular graphics.
Module: Teoria
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Module: Laboratorio
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This topics are part of the Biocrystallography oral exam
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