Publications

Authors:

Negri, S; Gambini, S; Ceoldo, S; Avesani, L; Commisso, M; Guzzo, F

Title:

Undifferentiated In Vitro Cultured Actinidia deliciosa as Cell Factory for the Production of Quercetin Glycosides

Year:

2021

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

PLANTS

ISSN of journal:

2223-7747

N° Volume:

10

Number or Folder:

11

Page numbers:

2499-2513

Keyword:

quercetin; flavonoids; plant cell and tissue culture; Actinida deliciosa; Actinidia chinensis

Short description of contents:

Land plants produce a vast arsenal of specialized metabolites and many of them display interesting bioactivities in humans. Recently, flavonol quercetin gained great attention in the light of the COVID-19 pandemic because, in addition to the anti-inflammatory, antiviral and anti-cancer activity already described, it emerged as possible inhibitor of 3CLpro, the major protease of SARS-CoV-2 virus. Plant cell and tissue culture (PCTC) is an attractive platform for the biotechnological production of plant metabolites. This technology allows a large amount of water and agricultural land to be saved and, being free of contaminants in the process, it is suitable for scaling up the production in bioreactors. In a project aimed to generate and screen in vitro plant cells for the production of valuable specialized metabolites for commercial production, we generated various cell lines from Actinidia deliciosa (kiwi fruit tree) and Actinidia chinensis (gold kiwi fruit tree), that were able to produce relevant amounts of quercetin derivatives, mainly quercetin glycosides. Three cell lines from A. deliciosa were characterized by targeted and untargeted metabolomics. In standard growing conditions, they produce and accumulate up to 13.26 mg/100 g fresh weight (419.76 mg/100 g dry weight) of quercetin derivatives. To address future industrial applications, these cell lines should be entered into an acceleration program to further increase the amount of these metabolites by optimizing the culture conditions and elicitation.

Product ID:

123800

Handle IRIS:

11562/1054032

Last Modified:

February 23, 2023

Bibliographic citation:

Negri, S; Gambini, S; Ceoldo, S; Avesani, L; Commisso, M; Guzzo, F, Undifferentiated In Vitro Cultured Actinidia deliciosa as Cell Factory for the Production of Quercetin Glycosides «PLANTS» , vol. 10 , n. 11 , 2021 , pp. 2499-2513

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo