Publications

Authors:

D'Incà, Erica; Foresti, Chiara; Orduña, Luis; Amato, Alessandra; Vandelle, Elodie; Santiago, Antonio; Botton, Alessandro; Cazzaniga, Stefano; Bertini, Edoardo; Pezzotti, Mario; Giovannoni, James; Vrebalov, Julia; Matus, José Tomás; Tornielli, Giovanni Battista; Zenoni, Sara

Title:

The transcription factor VviNAC60 regulates senescence- and ripening-related processes in grapevine

Year:

2023

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

Plant Physiology

ISSN of journal:

0032-0889

:

ASSPP

Page numbers:

1-19

Keyword:

DAP-seq; NAC transcription factors; grapevine; maturation; phase transition; senescence

Short description of contents:

: Grapevine (Vitis vinifera L.) is one of the most widely cultivated fruit crops because the winemaking industry has huge economic relevance worldwide. Uncovering the molecular mechanisms controlling the developmental progression of plant organs will prove essential for maintaining high-quality grapes, expressly in the context of climate change, which impairs the ripening process. Through a deep inspection of transcriptomic data, we identified VviNAC60, a member of the NAC transcription factor family, as a putative regulator of grapevine organ maturation. We explored VviNAC60 binding landscapes through DNA affinity purification followed by sequencing and compared bound genes with transcriptomics datasets from grapevine plants stably and transiently overexpressing VviNAC60 to define a set of high-confidence targets. Among these, we identified key molecular markers associated with organ senescence and fruit ripening. Physiological, metabolic, and promoter activation analyses showed that VviNAC60 induces chlorophyll degradation and anthocyanin accumulation through the up-regulation of STAY-GREEN PROTEIN 1 (VviSGR1) and VviMYBA1, respectively, with the latter being up-regulated through a VviNAC60-VviNAC03 regulatory complex. Despite sharing a closer phylogenetic relationship with senescence-related homologues to the NAC transcription factor AtNAP, VviNAC60 complemented the non-ripening(nor) mutant phenotype in tomato (Solanum lycopersicum), suggesting a dual role as an orchestrator of both ripening- and senescence-related processes. Our data support VviNAC60 as a regulator of processes initiated in the grapevine vegetative- to mature-phase organ transition and therefore as a potential target for enhancing the environmental resilience of grapevine by fine-tuning the duration of the vegetative phase.

Product ID:

132739

Handle IRIS:

11562/1088047

Last Modified:

December 20, 2024

Bibliographic citation:

D'Incà, Erica; Foresti, Chiara; Orduña, Luis; Amato, Alessandra; Vandelle, Elodie; Santiago, Antonio; Botton, Alessandro; Cazzaniga, Stefano; Bertini, Edoardo; Pezzotti, Mario; Giovannoni, James; Vrebalov, Julia; Matus, José Tomás; Tornielli, Giovanni Battista; Zenoni, Sara, The transcription factor VviNAC60 regulates senescence- and ripening-related processes in grapevine «Plant Physiology» , 2023 , pp. 1-19

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo