Testing drug resistance/sensitivity in cancer cell lines by Fourier Transform InfraRed microspectroscopy (microFTIR) and unsupervised pattern recognition.

Data inizio
4 maggio 2011
Durata (mesi) 
Responsabili (o referenti locali)
Bellisola Giuseppe

To demonstrate that microFTIR combined with unsupervised multi-parametric analysis in spectra is a robust method to achieve rapid and accurate information on drug-sensitivity and drug-resistance in leukemic cells. The FTIR absorbance spectra of single Ba/F3 cells expressing the drug-sensitive p210BCR-ABL or the drug-resistant p210T315IBCR-ABL mutant exposed to drugs, and of blast cells isolated from patients with Chronic Myeloid Leukemia (CML) responsive and non responsive to common anti-leukemic treatment protocols will be obtained. To identify drug-sensitive and drug-resistant cells, we will apply cluster analysis (CA) to selected intervals of frequencies where signatures of interference with BCR-ABL are expected in the IR spectrum of cells exposed to different drugs. Cross-validation will be obtained by complementary analyses in samples and correlation studies will be carried out.

Partecipanti al progetto

Giuseppe Bellisola
Elisabetta Moratti

Collaboratori esterni

Gianfelice Cinque
Diamond Light Source - UK B22 IR beamlline Principal Beamline Sientist
Sara Redaelli
Università di Milano-Bicocca Clinical Medicine and Prevention ricercatrice