Genomes - STRUTTURA E FUNZIONE DEI GENOMI (2014/2015)

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Course code
4S00291
Credits
6
Other available courses
Other available courses
Other available courses
    Academic sector
    BIO/18 - GENETICS
    Language of instruction
    Italian
    Location
    VERONA
    Teaching is organised as follows:
    Activity Credits Period Academic staff Timetable
    Teoria 1 5 I sem. Massimo Delledonne
    Teoria 2 1 I sem. Massimo Delledonne

    Lesson timetable

    Learning outcomes

    OBJECTIVES
    The course structure and function of genomes aims to provide the student with knowledge about the structure of eukaryotic and prokaryotic genomes, and methods of global analysis of genomes and transcriptomes. It also provides information about the replication and evolution of genomes, with particular emphasis on mecchanisms that regulate this activity.


    CONTENTS
    The genome projects: organization and goals of the Human Genome Project, plants and animals genomes. Mapping, sequencing and assembly of genomes. Interpretation of genomic sequences and global analysis of gene expression. Anatomy of eukaryotic and prokaryotic genomes. Operation of genomes and regulation of their activities. Replication and evolution of genomes. molecular phylogenesis

    Syllabus

    1- Genome mapping (dispense)
    Genetic and physical maps
    Molecular markers
    Gene tagging
    Tail PCR
    AIMS

    2 - Trancriptome analysis (dispense)
    cDNA
    Northern blot
    RT-PCR
    Real Time RT PCR
    cDNA libraries
    RACE (rapid amplification of cDNA ends)
    Subtractive libraries
    Reverse Northern
    Differential display / CDNA-AFLP
    SAGE (Serial Analysis of Gene Expression)
    EST and electronic northern
    Microarray
    RNA-Seq

    3 - Epigenetics (articoli)
    DNA methylation
    Histone modifications
    Methods to analyze methilated DNA

    4 - Genome analysis (articoli)
    DNA sequencing methods
    Genome assembly
    Localization of genes in a genomic sequence
    Functional characterization of genes (in silico and in vivo)
    Forward and Reverse genetics
    Genome wide association studies
    Personal genomics (molecular diagnostics)

    5 - Structure, function and evolution of plant and animal genomes (articoli)
    The Arabidopsis geneome and the 1001 genomes project
    The human genome (IHGC, Celera) and the 1000 genomes project
    The “minimal genome” project
    The ENCODE project
    Human genome interpretation(Ingenuity, Knome etc.)


    Suggested Text books: Genomi 3 EdiSES ISBN 978 88 7959 431 8
    Supplemental material is available here: http://profs.sci.univr.it/delledonne/Insegnamenti/Index.html

    Assessment methods and criteria

    Written examination. Open questions

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