Grape Biotechnology  and  Genomics (2020/2021)

Course code
4S001016
Credits
6
Coordinator
Sara Zenoni
Academic sector
AGR/07 - AGRICULTURAL GENETICS
Language of instruction
Italian
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
Teoria 5 II semestre Sara Zenoni

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Laboratorio 1 II semestre Sara Zenoni

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Learning outcomes

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MM: Teoria
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The course aims to provide the student with the knowledge and tools necessary for the application of the latest molecular technologies for assisted evolution of the vine. Basic knowledge will be provided for the study of gene expression, its regulation and the biological role of gravine genes. The tools necessary for the interpretation of the molecular mechanisms underlying the development of the plant and its responses to the environment will be illustrated. The student will hang on the importance of knowledge of the genome, of the function of the genes contained in it and of the progress that has occurred in the biotechnological field in recent years. The main stages of plant biotechnology will be illustrated, including in vitro cell cultures, protoplast isolation, plant regeneration by somatic embryogenesis, genetic transformation and genome editing. Particular emphasis will be given to the possible applications of these new technologies for the development of a modern viticulture able to respond more quickly and effectively to the stresses to which the plant is subjected.
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MM: Laboratorio
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The main objectives of the laboratory experience are: learning the main steps of the regeneration procedures of vine plants from single cells and related critical issues, knowledge of the main transformation protocols of vine cells with genes of interest and the procedures to conduct a molecular analysis on lives in order to study gene expression.

Syllabus

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MM: Teoria
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The genetic improvement of the vine: historical notes The study of gene expression and the function of genes in the vine Transcriptomics and its tools Transcriptomics applied to the study of vine biology Genomics and its tools The vine genome, history and results Biotechnology in the study of grapevine biology and interaction with the environment In vitro cultures of plant cells Vine somatic embryogenesis The isolation of protoplasts The genetic transformation of the vine Genome editing allied to the vine The future of biotechnological applications in viticulture
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MM: Laboratorio
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Obtaining and handling of embryogenic vine material in sterile conditions Transformation of vine cells in transient mode Molecular approaches for the study of gene expression in grapevines

Assessment methods and criteria

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MM: Teoria
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Learning will be assessed by an oral exam for both attending and non-attending students. The aim of the test is to ascertain the extend and the deepness of the the knowledge of the topics covered in the program. The final assessment is expressed in thirtieths
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MM: Laboratorio
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A laboratory experience report will be requested and evaluated for the final grade