Mid-Infrared Fourier Transform (FT-IR) spectroscopy and micro-spectroscopy, often coupled to multivariate statistical analysis techniques, is a rapid, accurate and non-destructive technique for the structural and molecular characterization of biological systems and metabolic processes. It allows obtaining information about the biochemical components (proteins, lipids, carbohydrates) with none or minimum sample preparation, hence without affecting the natural biological variability. This course aims at introducing the physical principles and some recent innovative applications of FT-IR spectroscopy including the study of biochemical processes in yeast and plant cells and cell walls and the characterization of biosynthesized selenium and tellurium nanoparticles. The course includes an experimental session at the laboratory of infrared spectroscopy and micro-spectroscopy (Lab IRIS - Infra-Red for Interdisciplinary Studies).
Principles of Infrared spectroscopy:
-Molecular vibrations and corresponding frequencies
-The Lambert-Beer law
-Mid-infrared absorption spectra: experimental techniques
-Fourier Transform Spectroscopy
-Infrared micro-spectroscopy and imaging
Applications to biotechnological research:
-Autolysis of wine yeasts
-Bio-reduction of toxic metal ions into bio-nanoparticles
-Role of expansin in transgenic petunia
-Ripening and post-harvest withering of Corvina berries
Data analysis techniques:
-Curve-fitting
-Multivariate statistical analysis: HCA, PCA and Heatmaps
No exam required
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