This course aims to provide the students with basic and advanced knowledge in the biochemical field for understanding the catalytic potential of enzymes and their use for innovative biotechnological applications, mainly in the industrial field. In particular, some objectives of the course concern the understanding: 1) of the approaches for the production and use of enzymes at industrial scale; 2) the main techniques used for the purification, analysis and storage of enzymes; 3) of the methodologies used to develop robust enzymes for industrial applications and 4) of the chemo-enzymatic industrial processes for the production of value-added chemicals. In addition, the course will also provide examples of industrial applications for the production of therapeutics and molecules with diagnostic interest.
The detailed course syllabus is currently under construction. The topics listed below are indicative of the syllabus content and may be subject to changes by the lecturer:
-Introduction to the course and context
-Development of enzymes for industrial applications
-Production, purification and formulation of enzymes at industrial scale
- Principles of enzyme kinetics
-Enzymes for food production: bread, cheese, wine, beer, meat, fruit juices, soft drinks, etc.
-Enzymes for feed optimization: Amylase, Protease Cellulase, Xylansis and Phytase
-Enzymes for the production of biofuels (bioethanol and biodiesel): Cellulase, Hemicellulase, Laccase (and other oxygenases), LPMOs, Lipase and Esterase
-Enzymes used in the paper industry
-Enzymes in household and industrial detergents
-Enzymes in the textile and tanning industry
-Enzymes in cosmetics and personal care products
- Environmental applications of enzymes
-Enzymes for the production of chemical compounds and active ingredients
The final assessment aims to verify the achievement of the "Learning Outcomes" related to the topics reported in the "Course syllabus".
The final assessment consists of an oral dissertation aimed at ascertaining both that the student has acquired the knowledge defined in the course syllabus as well as the ability to perform the required logical-deductive links. In particular, the completeness of the exposition, the level of integration between the various course topics as well as the scientific appropriateness of the language will be evaluated.
Furthermore, the achievement by the student of a global vision of the topics addressed in class (combined with their critical application), the ability to make connections and the use of an appropriate scientific language will be assessed with a mark of excellence.
The final assessment follows the same guidelines for both attending and non-attending students.