|teoria||5||I semestre||Daniela Cecconi|
|laboratorio||1||I semestre||Daniela Cecconi|
The aim of clinical proteomics is actually to find molecular signatures, to describe affected pathways and possibly identify candidate biomarkers that can help in the diagnosis, prognosis and prediction of therapeutic outcomes and elucidate pathogenic mechanisms.
Upon completing the course, students will have the knowledge necessary to recognize the strengthens and weakness of the different proteomics methodologies and of their application to current areas of clinical investigation.
The student will acquire laboratory skills through a series of experiments concerning the proteomic analysis through two-dimensional electrophoresis of biological samples.
• Introduction to clinical proteomics
• Strategies for protein sample preparation
• Gel-based and gel-free clinical proteomics analyses
• Protein Identification by Tandem Mass Spectrometry
• Label-Based and Label-free MS clinical proteomic approaches
• Differential profiling of Breast Cancer plasma proteome for biomarkers identification
• Clinical Proteomics to study Pancreatic Cancer Stem Cells
• Brain tissue proteomic analysis to identify biomarkers of Alzheimer’s Disease
• Evaluation of therapeutic effects of neural stem cells therapy in Parkinson’s disease
• Proteomics of cerebrospinal fluid to identify biomarkers for amyotrophic lateral sclerosis
• Pharmacoproteomics for elucidating the mechanism of action of anticancer drugs
The student will participate to practical experiences which are typical analyzes of a clinical proteomics laboratory, such as: preparation of a protein extract for proteomic analysis, separation by isoelectric-focusing of proteins, separation by two-dimensional electrophoresis of protein extracts, staining and evaluation of proteomic profiles.
The aim of the exam is to verifying the level of achievement of the learning outcomes which are above indicated.
The exam consists of a written test that covers all the topics of the program. The students will have to answer to different open questions, demonstrating that they understand, and are able to use, the basic concepts of each topic. Understanding of practical experiences will be verified through questions which are included in the exam.
The final vote is obtained from the following formula: Vote = Vote_written exam
|teoria||Josip Lovric||Introducing Proteomics: From concepts to sample separation, mass spectrometry and data analysis||Wiley||2011||978-0-470-03524-5|