Molecular biology (2019/2020)



Course code
4S00800
Credits
12
Coordinator
Daniele Guardavaccaro
Academic sector
BIO/11 - MOLECULAR BIOLOGY
Language of instruction
Italian
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
teoria 9 II semestre Daniele Guardavaccaro

Go to lesson schedule

laboratorio [1° turno] 3 II semestre Daniele Guardavaccaro

Go to lesson schedule

laboratorio [2° turno] 3 II semestre Daniele Guardavaccaro

Go to lesson schedule

Learning outcomes

The aim of this course is to give the students the basic knowledge of the molecular mechanisms concerning transmission, variation and expression of the genetic information.
At the end of the course the students will be able to recognize the major molecular mechanisms of life in prokaryotic and eukaryotic cells.



Syllabus

THEORY
DNA AND CHROMSOMES
The structure and function of DNA
Chromosomal DNA and its packaging in the chromatin fiber
Chromatin structure and function
The global structure of chromosomes
DNA REPLICATION, REPAIR AND RECOMBINATION
DNA replication mechanisms
The initiation and completion of DNA replication in chromosomes
DNA repair
Homologous recombination
Transposition
HOW CELLS READ THE GENOME: FROM DNA TO PROTEIN
From DNA to RNA
From RNA to protein
Protein folding, degradation and the ubiquitin-proteasome system
CONTROL OF GENE EXPRESSION
Control of transcription by sequence specific DNA-binding proteins
Transcription regulators
Molecular genetic mechanisms that create and maintain specialized cell types
Mechanisms reinforcing cell memory in plants and animals
Post-transcriptional controls
Regulation of gene expression by non-coding RNAs
ANALYZING CELLS AND MOLECULES
Isolating cells and growing them in culture
Analyzing and manipulating DNA
Studying gene expression and function
CELL SIGNALING
Principles of cell signaling
Signaling through G-protein-coupled receptors
Signaling through enzyme-coupled receptors
Alternative signalling routes in gene regulation
THE CELL CYCLE
Overview of the cell cycle
The cell cycle control system
S-phase
Mitosis
Cytokinesis
Meiosis
Control of cell division and cell growth
CANCER
Cancer as a microevolutionary process
Cancer-critical genes: oncogenes and tumor suppressors
Cancer therapy
DISCUSSION OF TOPICS CHOSEN FROM THE LITERATURE ON MOLECULAR BIOLOGY

PRACTICAL (video tutorials):
Restriction enzyme digestion
DNA gel electrophoresis
DNA purification from agarose gel
DNA ligation
Bacterial transformation (heat shock method)
Bacterial transformation (electroporation method)
Plasmid purification
Polymerase chain reaction
Immunoprecipitation, co-immunoprecipitation and pull-down assays
SDS-PAGE
Western blotting
Genetic screens
Genetic engineering of model organisms
Expression profiling with microarrays
RNA-seq
Chromatin immunoprecipitation (ChIP)

Assessment methods and criteria

Oral examination. The final exam consists of three questions concerning any of the topics treated during the course. It is passed if all answers are positive.

Reference books
Activity Author Title Publisher Year ISBN Note
teoria Jocelyn E. Krebs, Elliott S. Goldstein, Stephen T. Kilpatrick Lewin's Genes XII (Edizione 12) Jones & Bartlett Pub 2017 1284104494
teoria Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff Molecular Biology of the Cell (Edizione 6) Garland Science 2014 0815344643
teoria Nancy Craig, Rachel Green, Carol Greider, Gisela Storz, Cynthia Wolberger, Orna Cohen-Fix Molecular Biology: Principles of Genome Function (Edizione 2) OUP Oxford 2014 0199658579
teoria Arnold Berk, Chris A. Kaiser, Harvey Lodish, Angelika Amon, Hidde Ploegh, Anthony Bretscher, Monty Krieger, Kelsey C. Martin Molecular Cell Biology (Edizione 8) WH Freeman 2016 1464187444
teoria Erik Pierre Molecular Cloning (Edizione 1) Ml Books International 2015 1632394685
teoria Michael R. Green e Joseph Sambrook Molecular Cloning: A Laboratory Manual, Fourth Edition (Edizione 4) CSHL Press 2012 978-1-936113-42-2
teoria Geoffrey M. Cooper, Robert E. Hausman The cell: a molecular approach (Edizione 6) Sinauer Associates, Inc 2013 978-1-60535-155-1
Teaching aids
Title Format (Language, Size, Publication date)
Articoli e gruppi  pdfpdf (it, 60 KB, 12/05/20)
Ballas et al  pdfpdf (it, 1206 KB, 12/05/20)
Carrano et al  pdfpdf (it, 1079 KB, 12/05/20)
Cepeda et al  pdfpdf (it, 6333 KB, 12/05/20)
Choi et al  pdfpdf (it, 11191 KB, 12/05/20)
Duan2 et al  pdfpdf (it, 10278 KB, 12/05/20)
Duan et al  pdfpdf (it, 3841 KB, 12/05/20)
Jin et al  pdfpdf (it, 7501 KB, 12/05/20)
Koo et al  pdfpdf (it, 12248 KB, 12/05/20)
Lamers et al  pdfpdf (it, 8097 KB, 12/05/20)
Mamely et al  pdfpdf (it, 645 KB, 12/05/20)
Michel et al  pdfpdf (it, 1557 KB, 12/05/20)
Mohamed et al  pdfpdf (it, 8060 KB, 12/05/20)
Raducu et al  pdfpdf (it, 6537 KB, 12/05/20)
Strohmaier et al and Koepp et al  pdfpdf (it, 4440 KB, 12/05/20)
Zhang et al  pdfpdf (it, 3446 KB, 12/05/20)