|a||1||II sem.||Massimo Delledonne|
|b||1||II sem.||Paola Dominici|
|c||1||II sem.||Alejandro Giorgetti|
|d||1||II sem.||Daniela Cecconi|
|e||2||II sem.||Alessandra Maria Bossi|
The course is divided into 4 interdisciplinary laboratory modules focused on a topic of biological relevance. The main purpose of the course is to offer the student tools to focus on the problem, by using different highly complementary techniques. The module of PROTEIN ENGINEERING aims to provide to the students with specific information on the principles and techniques used in protein engineering, with particular reference to the production of recombinant proteins in heterologous systems (construction and expression of foreign gene in prokaryotic and eukaryotic host cell).
Definition of recombinant protein. Introduction to protein engineering. Acquisition of the required information (theoretical and experimental) to carry out the process of engineering of a protein function/structure. Production of recombinant proteins. Experimental approaches to study and modulate the protein functionality. Protein characterization (Site directed mutagenesis, Gel electrophoresis, Tryptophan (Trp) fluorescence, ANS Fluorescence, Limited proteolysis). Examples of application of protein engineering.
The verification of the acquisition of concepts and protocols inherent to the thematics of the research inspired laboratory , will be through a global exam, subdivided into 10 open questions based on the 5 modules (2 questions for bioinformatics; 2 for biochemistry; 2 for expressional proteomics, 2 for genetics and 3 for functional proteomics) to be replied in 3 hours.
All the questions aims at verifying acquisition of the knowledge of the practicals and of the inherent theories discussed over the course.