Analytical Techniques for Clinical Chemistry (2015/2016)

Course code
Daniela Cecconi
Academic sector
Language of instruction
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
teoria 5 II semestre Daniela Cecconi
laboratorio 1 II semestre Daniela Cecconi

Lesson timetable

II semestre
Activity Day Time Type Place Note
teoria Tuesday 10:30 AM - 12:30 PM lesson Lecture Hall H  
teoria Thursday 2:30 PM - 4:30 PM lesson Lecture Hall F from Apr 7, 2016  to Jun 10, 2016
laboratorio Wednesday 2:30 PM - 7:30 PM laboratorio Laboratory Laboratorio di Chimica Fisica from May 25, 2016  to May 25, 2016
laboratorio Thursday 2:30 PM - 7:30 PM laboratorio Laboratory Laboratorio di Chimica Fisica from May 26, 2016  to May 26, 2016
laboratorio Friday 2:30 PM - 7:30 PM laboratorio Laboratory Laboratorio di Chimica Fisica from May 27, 2016  to May 27, 2016

Learning outcomes

The course aims to provide students with the basic principles for the interpretation of analytical data and the reliability of analytical measurements (errors, preanalytical and analytical variability, precision, accuracy, sensitivity and specificity, prediction).
The most important analytical techniques for clinical chemistry will be described (such as techniques for the enzymatic determination of analyte concentration, immunochemical techniques, radiochemical and electrochemical techniques, immunoelectrophoresis). The potential and weaknesses of the various techniques will be illustrated by examples of determination of biological samples.



Collection of biological materials.
The components of the variability in clinical chemistry (pre-analytical, biological, analytical). Experimental errors.
Precision, accuracy, sensitivity and specificity of analytical measurements.
Predictability of an analytical test. Quality control of the results.
Techniques for the detection of enzyme activity. Enzymatic determination of analyte concentration.
Immunochemical techniques. Heterogeneous (competitive / non-competitive), homogeneous (competitive / non-competitive).
Immunoenzymatic assays (Enzyme Linked Immunosorbent Assay; Microparticle enzyme immunoassay; Enzyme Multiplied immunoassay; Cloned enzyme donor immunoassay).
Immunofluorescent techniques (Immunofluorescence Assay, Fluorescence Polarization immunoassay; Dissociation-Enhanced Lanthanide Fluorescent immunoassay; IF Microscopy and FACS).
Chemiluminescence (Lumino immunoassay; Chemiluminescent Magnetic immunoassay; ElectroChemiLuminiscence; Bioluminescence enzyme immunoassay; Immunoassays with avidin-biotin)
Radioimmunoassay (Radio immunoassay; Immuno Radiometric Assay).
Agglutination test. Complement fixation test.
Immunodiffusion; Immunoelectrophoresis; Counter current electrophoresis.
Immunonephelometry and Immunoturbidimetry.
Electrolytes and electrochemical techniques in diagnostics.

Assessment methods and criteria


The final exam includes the presentation of a scientific paper related to one of the techniques discussed during the course and a written exam that will cover all the topics of the program. The student will have to answer a series of open questions, demonstrating that they understand and be able to use the basic concepts of each topic.