|teoria||3||II semestre||Michael Assfalg|
|laboratorio||1||II semestre||Michael Assfalg|
|teoria||Friday||10:30 AM - 1:30 PM||lesson||Lecture Hall H|
Metabolomics is the systematic and holistic study of metabolites present in an organism or a sample. The purpose of the course is to introduce metabolomic analysis. We address the analytical techniques used in the field. We will then present the physical principles that underlie the technique of nuclear magnetic resonance and discuss the methods of multivariate analysis applied to spectroscopic data. The course also aims to provide students with an overview of the applications of metabolomics in different areas.
Introduction. Terminology and definitions. The chemical complexity of the metabolome. Applications and purposes of metabolomics. Metabolomic approaches . The analytical techniques. Chromatography. Mass spectrometry.
NMR spectroscopy. Physical principles. Spin number. Energy levels. Sensitivity. Precession. Angular frequency. Magnetization. Oscillating magnetic field. Rediofrequency pulses. Fourier transform.
Apodization functions. Spectral windows. Chemical shift. Two-dimensional spectroscopy.
Chemical shift: shielding constant, diamagnetic, paramagnetic and local contributions. Electronegativity, mesomeric effects, anisotropic contributions of bonds and aromatic rings. Scalar coupling constant, multiplets. 1D spectrum of alanine from the BMRB database .
Methods of suppression of the signal of solvent. 1D 13C - alanine from the BMRB. Two-dimensional spectroscopy : heterocorrelation , J -resolved, COSY , TOCSY .
Multivariate analysis. Examples of applications in analysis of food juice , oil, beer, tea, others.
Exercises for the interpretation of NMR spectra of organic molecules
Practical notes on the recording of spectra : sample preparation, temperature control , lock , tuning, shimming, calibration pulse, number of scans.
Preparation of samples of food fluids.
Execution of proton spectra.
Analysis and interpretation of the spectra with the aid of spectral databases.
Multivariate analysis of the recorded spectra
|Title||Format (Language, Size, Publication date)|
|Articolo_Cromatografia||pdf (it, 275 KB, 31/05/14)|
|Articolo_GCMS||pdf (it, 729 KB, 31/05/14)|
|Articolo_LCMS||pdf (it, 139 KB, 31/05/14)|
|Articolo_NMR||pdf (it, 1619 KB, 31/05/14)|
|Articolo_Statistica||pdf (it, 2832 KB, 31/05/14)|
|DomandeTipo1||pdf (it, 37 KB, 31/05/14)|
|DomandeTipo2||pdf (it, 922 KB, 31/05/14)|
|Lezione1||pdf (it, 4556 KB, 18/03/14)|
|Lezione2||pdf (it, 414 KB, 18/03/14)|
|Lezione3||pdf (it, 204 KB, 21/03/14)|
|Lezione4||pdf (it, 3406 KB, 27/03/14)|
|Lezione5||pdf (it, 2087 KB, 11/04/14)|
|Lezione6||pdf (it, 6335 KB, 30/05/14)|
|Programma||pdf (it, 26 KB, 21/03/14)|