|teoria||1||II semestre||Diana Bellin|
|laboratorio||3||II semestre||Diana Bellin|
|teoria||Monday||2:30 PM - 4:30 PM||lesson||Lecture Hall L||from Apr 7, 2014 to Jun 13, 2014|
|laboratorio||Monday||2:30 PM - 7:30 PM||lesson||Laboratory Laboratorio di Biotecnologie genetiche|
This course introduces to some new analytical technologies widely used for genome analysis, functional genomics and trascriptomics. The purpose is to provide both knowledge of theory for these technologies as well as practical experience.
-GATEWAY TECHNOLOGY: Lamba phage recombination and introduction to gateway technology, BP and LR reactions, ccdB based selection, ENTRY clone production, TOPO cloning, gateway compatible destination vectors.
-MUTAGENESIS: DNA mutations, mutagenesis in forward and inverse genetics, random mutagenesis (chemical or PCR based), site directed mutagenesis (with or without PCR), Quickchange, DNA shuffling
-RT-PCR AND REAL TIME RT-PCR: single gene expression analysis and genome-wide expression analysis, RT-PCR, Real Time RT-PCR, absolute and relative quantification (delta delta Ct method for data analysis), SYBR green analysis, TaqMan probes, LUX, Molecular Beacons and Scorpions
-MICROARRAY: cDNA microarray and their production, oligoarray and oligoarray production, direct and indirect probe labeling, experimental design and controls for microarray experiment setup, hybridization, image acquisition and data analysis
Gene Cloning using the gateway technology and transfer of a gene in a destination vector
Expression analysis using real time RT-PCR
Microarray expression analysis
See main page of the integrated course